Sheikh K M, Gahan P B
Histochem J. 1976 Jan;8(1):87-92. doi: 10.1007/BF01004009.
Soluble proteins isolated from tissues of the tomato Lycopersicon esculentum, after inoculation with Agrobacterium tumefaciens to induce tumours, have been examined by gel electrophoresis and cytochemically. Changes that occur include the suppression of host enzymes, the appearance of bacterial enzymes in the host tissues and the appearance of new enzyme bands in the affected cells. These changes are detectable within 6 hr of infection and prior to evident morphological changes, and may be explained as derepression and repression of host genes, or the expression of released bacterial genes in the host cells.
从番茄(Lycopersicon esculentum)组织中分离出的可溶性蛋白质,在用根癌土壤杆菌接种诱导肿瘤后,已通过凝胶电泳和细胞化学方法进行了检测。发生的变化包括宿主酶的抑制、宿主组织中细菌酶的出现以及受影响细胞中新酶带的出现。这些变化在感染后6小时内且在明显的形态变化之前即可检测到,并且可以解释为宿主基因的去抑制和抑制,或者是宿主细胞中释放的细菌基因的表达。