Lanan M, Shick R, Morris M D
Department of Chemistry, University of Michigan, Ann Arbor 48109-1055.
Biopolymers. 1991 Aug;31(9):1095-104. doi: 10.1002/bip.360310909.
Time-resolved and steady-state electric birefringence imaging with a slow-scan video camera is used to study orientation during DNA agarose gel electrophoresis. The hydrodynamically induced gel distortion is shown to be the major source of birefringence under electrophoresis running conditions and to generate a birefringence image that approximates the image of the DNA concentration gradient in the electric field direction. A fluid kinematic model is presented to describe the spatial distribution of steady-state birefringence and is verified with fluorescence measurements of DNA distribution. The stress-optic coefficient of 1% agarose gel is measured by mechanical compression and used to evaluate the magnitude of the induced strain on the gel during electrophoresis.
利用带有慢扫描摄像机的时间分辨和稳态电双折射成像技术研究DNA琼脂糖凝胶电泳过程中的取向。结果表明，在电泳运行条件下，流体动力学引起的凝胶变形是双折射的主要来源，并产生一个近似于电场方向上DNA浓度梯度图像的双折射图像。提出了一个流体运动学模型来描述稳态双折射的空间分布，并用DNA分布的荧光测量进行了验证。通过机械压缩测量了1%琼脂糖凝胶的应力-光学系数，并用于评估电泳过程中凝胶上诱导应变的大小。
