Burlakov G V, Kamysheva V A
Biull Eksp Biol Med. 1991 Aug;112(8):170-4.
Research on isolated hearts of Rana temporaria has shown that upon treating them with anti-cholinoreceptor antibodies, there occurs a considerable reduction of inhibition effect on cardiac activity of acetylcholine or carbacholine. A reduction of inhibition effect was noticed after incubation of frog's heart with antibodies against cholinoreceptors obtained from motor-denervated muscles of frogs as well as from muscles of mice Balb/c. Cholinoreceptor protein was obtained and purified by A. Sobel's method. Control tests were made with serum of non-immunized rabbits and rabbits immunized with material obtained from non-denervated muscles of frogs. It was concluded that acetylcholinoreceptor antibodies are capable of provoking atropine-like effect on frog's heart. According to our data, anti-cholinoreceptor antibodies as well as cholinoreceptors are relatively non-specific to species.
对林蛙离体心脏的研究表明，用抗胆碱能受体抗体处理这些心脏后，乙酰胆碱或卡巴胆碱对心脏活动的抑制作用会显著降低。在用从青蛙运动去神经肌肉以及Balb/c小鼠肌肉中获得的抗胆碱能受体抗体孵育青蛙心脏后，观察到抑制作用降低。胆碱能受体蛋白是通过A. 索贝尔的方法获得并纯化的。用未免疫兔子的血清以及用从青蛙未去神经肌肉中获得的物质免疫的兔子血清进行了对照试验。得出的结论是，乙酰胆碱能受体抗体能够对青蛙心脏产生类似阿托品的作用。根据我们的数据，抗胆碱能受体抗体以及胆碱能受体对物种的特异性相对较低。
