一种用于测定某些糖原中链长分布的高效液相色谱法。

An h.p.l.c. method for determining chain-length distribution in some glycogens.

作者信息

Cheetham N W, Hansawek N, Saecou P

机构信息

School of Chemistry, University of New South Wales, Kensington, Sydney, Australia.

出版信息

Carbohydr Res. 1991 Aug 12;215(1):59-65. doi: 10.1016/0008-6215(91)84007-2.

DOI:10.1016/0008-6215(91)84007-2

PMID:1786580

Abstract

Human, oyster, Streptococcus mitis, and phyto-glycogen samples were debranched using Pseudomonas amylodermosa isoamylase (EC 3.2.1.68). The distribution of chain lengths was studied by high-performance liquid chromatography on reversed-phase columns, with water as eluent. Quantitative data was obtained over the degree of polymerisation range three to eighteen (d.p. 3-18), and oligosaccharides up to d.p. 26 were detected. No single column was found suitable for the resolution of the complete range of oligosaccharides, two columns being necessary for the quantitative analysis. The resulting "fingerprints" of chain lengths are characteristic of the glycogen source and should be useful for both comparison purposes among glycogens and for monitoring procedures of glycogen isolation.

摘要

使用解淀粉假单胞菌异淀粉酶（EC 3.2.1.68）对人、牡蛎、缓症链球菌和植物糖原样品进行脱支处理。通过在反相柱上进行高效液相色谱，以水为洗脱剂，研究链长分布。在聚合度范围为3至18（d.p. 3 - 18）内获得定量数据，并检测到聚合度高达26的寡糖。未发现单一色谱柱适合分离整个寡糖范围，定量分析需要两根色谱柱。所得的链长“指纹图谱”是糖原来源的特征，对于糖原之间的比较以及糖原分离的监测程序都应是有用的。