Vogel M W, McInnes M, Zanjani H S, Herrup K
Maryland Psychiatric Research Center, Baltimore 21228.
Brain Res Dev Brain Res. 1991 Dec 17;64(1-2):87-94. doi: 10.1016/0165-3806(91)90212-2.
The distribution of Purkinje cells, granule cells, and olivary neurons was quantitatively analyzed in a lurcher +/Lc in equilibrium C3H/HeJ chimera in which the surviving wild type Purkinje cells were unilaterally distributed in the left hemicerebella. The left hemisphere of this mouse contains 7600 Purkinje cells, approximately 10% of the number of Purkinje cells in inbred C3H/HeJ mice. The right hemisphere contains 300 Purkinje cells, all of which are found within 200 microns of the midline. As in other +/Lc in equilibrium wild type chimeras, the ratio of granule cells to Purkinje cells is increased in the left hemisphere, reflecting increased granule cell survival. In the right hemisphere, however, the number of granule cells is reduced to that found in +/Lc mutants. In the inferior olive, almost twice as many neurons are found in the right nucleus as opposed to the left nucleus. As the projections of olivary neurons are crossed, the number of olivary neurons is increased in the nuclei that project to the cerebellar hemisphere containing Purkinje cells compared to the olivary nuclei that project to the cerebellar hemisphere with almost no Purkinje cells. The preferential survival of granule cells and olivary neurons that either occupy or project to the hemicerebellum containing Purkinje cells suggests that the availability of trophic support from target Purkinje cell neurons is spatially restricted.
在一只处于平衡状态的lurcher +/Lc C3H/HeJ嵌合体小鼠中，对浦肯野细胞、颗粒细胞和橄榄核神经元的分布进行了定量分析，在该嵌合体中，存活的野生型浦肯野细胞单侧分布于左半小脑。这只小鼠的左半球含有7600个浦肯野细胞，约为近交系C3H/HeJ小鼠浦肯野细胞数量的10%。右半球含有300个浦肯野细胞，所有这些细胞都位于中线200微米范围内。与其他处于平衡状态的+/Lc野生型嵌合体一样，左半球颗粒细胞与浦肯野细胞的比例增加，反映出颗粒细胞存活率提高。然而，在右半球，颗粒细胞数量减少到+/Lc突变体中的水平。在延髓下橄榄核中，右核中的神经元数量几乎是左核中的两倍。由于橄榄核神经元的投射是交叉的，与投射到几乎没有浦肯野细胞的小脑半球的橄榄核相比，投射到含有浦肯野细胞的小脑半球的核中，橄榄核神经元数量增加。占据或投射到含有浦肯野细胞的半小脑的颗粒细胞和橄榄核神经元优先存活，这表明来自靶浦肯野细胞神经元的营养支持的可用性在空间上受到限制。
