Wolfe M W, Stumpf T T, Roberson M S, Kittok R J, Kinder J E
Department of Animal Science, University of Nebraska, Lincoln 68583.
Domest Anim Endocrinol. 1991 Oct;8(4):491-8. doi: 10.1016/0739-7240(91)90018-f.
The objective of the present study was to examine the involvement of opioid neuropeptides and E2 in regulating circulating concentrations of gonadotropins during sexual maturation in the bovine female. Prepubertal (immature) and postpubertal (mature) bovine females were used. Mean concentrations of luteinizing hormone (LH) and follicle-stimulating hormone (FSH) in circulation before and after administration of naloxone were determined in ovariectomized heifers administered E2 and ovariectomized heifers not administered E2. A linear decline (P less than 0.01) in opioid suppression of LH and FSH occurred during the experimental period in immature heifers receiving E2. This decline in opioid suppression of LH and FSH occurred during the same period of time that intact control heifers were initiating estrous cycles at puberty. Little change of opioid suppression of LH and FSH occurred during the experimental period in immature heifers not receiving E2 and mature heifers receiving E2. Our research indicates that opioid neuropeptides and E2 act together to regulate LH and FSH secretion during sexual maturation in the bovine female.
本研究的目的是检测阿片类神经肽和雌二醇（E2）在调节雌性牛性成熟过程中促性腺激素循环浓度方面的作用。选用青春期前（未成熟）和青春期后（成熟）的雌性牛。在给予E2的去卵巢小母牛和未给予E2的去卵巢小母牛中，测定给予纳洛酮前后循环中促黄体生成素（LH）和促卵泡生成素（FSH）的平均浓度。在接受E2的未成熟小母牛的实验期间，阿片类物质对LH和FSH的抑制作用呈线性下降（P<0.01）。LH和FSH的阿片类抑制作用下降发生在完整对照小母牛在青春期开始发情周期的同一时间段。在未接受E2的未成熟小母牛和接受E2的成熟小母牛的实验期间，LH和FSH的阿片类抑制作用几乎没有变化。我们的研究表明，阿片类神经肽和E2共同作用，调节雌性牛性成熟过程中LH和FSH的分泌。
