Gaál T, Halmay Dóra, Kocsis R, Abonyi-Tóth Z
Department of Internal Medicine Faculty of Veterinary Science, Szent István University, H-1078 Budapest, István u. 2, Hungary.
Acta Vet Hung. 2007 Sep;55(3):287-94. doi: 10.1556/AVet.55.2007.3.2.
The effect of two nonsteroidal anti-inflammatory drugs (carprofen and ketoprofen) on platelet adhesion and aggregation functions was evaluated by the PFA-100 analyser (Dade-Behring, CA, U.S.A.) using its collagen-adenosine diphosphate (ADP) and collagen-epinephrine (EPI) cartridges. The function of platelets was evaluated in 55 healthy dogs, in 7 dogs treated with ketoprofen and in 31 dogs treated with carprofen in a therapeutic dose for minimum 5 days. The therapeutic doses of carprofen had no effect on the closure time of PFA-100 (which is the marker of platelet function) but ketoprofen caused a significant increase when using collagen-EPI stimulation The closure times for both the healthy (control) and the treated dogs using EPI cartridges were often longer than the upper default cut-off point (300 sec) of the device. The PFA-100 analyser with collagen-ADP cartridges could be a useful tool for veterinary applications including the evaluation of platelet aggregation in dogs treated with NSAIDs. The upper cut-off point of PFA-100 might be extended.
使用美国加利福尼亚州达德-拜林公司生产的PFA-100分析仪，通过其胶原-二磷酸腺苷（ADP）和胶原-肾上腺素（EPI）检测卡，评估两种非甾体抗炎药（卡洛芬和酮洛芬）对血小板黏附和聚集功能的影响。在55只健康犬、7只接受酮洛芬治疗的犬以及31只接受卡洛芬治疗且治疗剂量至少为5天的犬中评估血小板功能。卡洛芬的治疗剂量对PFA-100的封闭时间（血小板功能的标志物）没有影响，但在使用胶原-EPI刺激时，酮洛芬会导致封闭时间显著增加。使用EPI检测卡时，健康（对照）犬和治疗犬的封闭时间通常长于该设备的默认上限（300秒）。配备胶原-ADP检测卡的PFA-100分析仪可能是兽医应用中的一个有用工具，包括评估接受非甾体抗炎药治疗的犬的血小板聚集情况。PFA-100的上限可能需要延长。
