Fleming W A, McNeill T A
J Cell Physiol. 1976 Jul;88(3):323-9. doi: 10.1002/jcp.1040880308.
Addition of low concentrations (10 ng/ml) of saponin or Tween 80 to stimulated cultures of normal mouse bone marrow in agar increased the number of granulocyte-macrophage colonies which developed. Addition of cyclic AMP or dibutyryl cyclic AMP in low concentration (10(-8) to 10(-10) M) also enhanced colony numbers although concentrations above 10(-5) M were inhibitory. enhancement was found when marrow cells were pre-treated with these agents and cultured in their absence. The agents did not stimulate colony development in the absence of colony-stimulating factor and enhancement of colony number occurred only in cultures containing a concentration of colony-stimulating factor which was sub-optimal in terms of maximum colony development. There was no indication of increased colony-stimulating factor production by treated marrow cells under the experimental conditions used to show colony enhancement. It was concluded that the agents caused an increased responsiveness of colony-forming cells to colony-stimulating factor.
向琼脂中正常小鼠骨髓的刺激培养物中添加低浓度（10纳克/毫升）的皂角苷或吐温80，会增加所形成的粒细胞-巨噬细胞集落的数量。添加低浓度（10⁻⁸至10⁻¹⁰摩尔）的环磷酸腺苷或二丁酰环磷酸腺苷也会增加集落数量，尽管浓度高于10⁻⁵摩尔时具有抑制作用。当骨髓细胞用这些试剂预处理并在无这些试剂的情况下培养时，发现有增强作用。在没有集落刺激因子的情况下，这些试剂不会刺激集落形成，并且集落数量的增加仅发生在含有集落刺激因子浓度低于最大集落形成所需最适浓度的培养物中。在所使用的显示集落增强的实验条件下，没有迹象表明经处理的骨髓细胞会增加集落刺激因子的产生。得出的结论是，这些试剂导致集落形成细胞对集落刺激因子的反应性增加。
