Takahashi Yosuke, Shibata Toshihiko, Sasaki Yasuyuki, Hirai Hidekazu, Takemura Shigekazu, Minamiyama Yukiko, Sakaguchi Masanori, Suehiro Shigefumi
Department of Cardiovascular Surgery, Osaka City University, Graduate School of Medicine, Japan.
Osaka City Med J. 2007 Jun;53(1):9-16.
This study was designed to investigate whether administration of landiolol before or during ischemia protects the myocardium against ischemia-reperfusion (I/R) injury.
Isolated rat hearts were perfused with Krebs-Henseleit buffer using Langendorff apparatus. Cardiac arrest was achieved using St. Thomas' Hospital cardioplegic solution for 3 min. Each heart was subjected to global ischemia at 37 degrees C for 45 min followed by reperfusion for 60 min. In protocol A, landiolol (5-200 microM) was administered before ischemia and in protocol B, landiolol (10-1000 microM) was administered during ischemia. Post-ischemic recovery of cardiac function after 60 min of reperfusion was expressed as a percentage of the pre-ischemic value. Creatinine kinase (CK) leakage during reperfusion for 20 min was also measured.
Post-ischemic recovery of cardiac function and CK leakage were significantly improved by administration of 100 microM landiolol in protocol A (left ventricular developed pressure, 54.5 +/- 10.5% vs 76.1 +/- 6.6%; dP/dtmax, 47.5 +/- 14.9% vs 71.4 +/- 6.9%; coronary flow, 68.5 +/- 7.1% vs 83.9 +/- 12.9%, and CK, 25.3 +/- 4.2 IU vs 20.3 +/- 7.4 IU). In protocol B, landiolol exerted no cardioprotective effect on cardiac function and CK leakage at each concentration.
Landiolol has a cardioprotective effect on I/R injury in the rat heart when administered before ischemia.
本研究旨在调查在缺血前或缺血期间给予兰地洛尔是否能保护心肌免受缺血再灌注（I/R）损伤。
使用Langendorff装置用Krebs-Henseleit缓冲液灌注离体大鼠心脏。用圣托马斯医院心脏停搏液使心脏停搏3分钟。每颗心脏在37℃下进行45分钟的全心缺血，然后再灌注60分钟。在方案A中，在缺血前给予兰地洛尔（5-200微摩尔），在方案B中，在缺血期间给予兰地洛尔（10-1000微摩尔）。再灌注60分钟后缺血后心脏功能的恢复以缺血前值的百分比表示。还测量了再灌注20分钟期间的肌酸激酶（CK）泄漏。
在方案A中，给予100微摩尔兰地洛尔可显著改善缺血后心脏功能的恢复和CK泄漏（左心室舒张末压，54.5±10.5%对76.1±6.6%；dP/dtmax，47.5±14.9%对71.4±6.9%；冠状动脉流量，68.5±7.1%对83.9±12.9%，以及CK，25.3±4.2国际单位对20.3±7.4国际单位）。在方案B中，各浓度的兰地洛尔对心脏功能和CK泄漏均无心脏保护作用。
缺血前给予兰地洛尔对大鼠心脏的I/R损伤具有心脏保护作用。
