Qiao Yanyun, Zou Fangdong, Wei Kun, Yue Bisong
Key Laboratory of Bio-Resources and Eco-Environment (Ministry of Education), College of Life Sciences, Sichuan University, Chengdu, Sichuan, PR China.
Zoolog Sci. 2007 May;24(5):493-5. doi: 10.2108/zsj.24.493.
We describe a rapid sex-identification method for the forest musk deer (Moschus berezovskii) using PCR based on zinc-finger protein-encoding genes (ZFX/ZFY) located on the X and Y chromosomes. Fragments of the ZFX and ZFY genes were amplified and sequenced. The ZFX and ZFY fragments were identical in length and 94% similar in nucleotide sequence. Specific primers for forest musk deer sex identification were designed on the basis of sequence differences between ZFX and ZFY. All the primers were multiplexed in single-tube PCR. Both male and female forest musk deer showed amplification bands of 447 bp and 212 bp separated in agarose gels. A sex-specific 278-bp band was amplified only from males. These results show that testing by PCR for the presence of the 278-bp sequence is a rapid and reliable method for sex identification.
我们描述了一种基于位于X和Y染色体上的锌指蛋白编码基因（ZFX/ZFY），利用PCR技术对林麝（Moschus berezovskii）进行快速性别鉴定的方法。扩增并测序了ZFX和ZFY基因的片段。ZFX和ZFY片段长度相同，核苷酸序列相似度为94%。根据ZFX和ZFY之间的序列差异设计了用于林麝性别鉴定的特异性引物。所有引物在单管PCR中进行多重反应。雄性和雌性林麝在琼脂糖凝胶中均显示出447 bp和212 bp的扩增条带。仅从雄性个体中扩增出一条278 bp的性别特异性条带。这些结果表明，通过PCR检测278 bp序列的存在是一种快速且可靠的性别鉴定方法。
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