Seok Seong Hoon, Kang Sun Young, Im Young Bin, Yoo Han Sang, Yeon Seong-Chan
Daegu Animal Medical Center, 36, Dongdaegu-ro, Suseong-gu, Daegu 42185, Republic of Korea.
Gyeongnam Wildlife Center, Gyeongsang National University, Jinju 52828, Republic of Korea.
J Vet Med Sci. 2019 May 31;81(5):793-798. doi: 10.1292/jvms.18-0693. Epub 2019 Apr 2.
In this study, we used multiplex polymerase chain reaction (PCR) to determine the sex of leopard cats (Prionailurus bengalensis euptilurus) in Korea. When we applied PCR using primers based on the intronic region between ZFX and ZFY, only one DNA band of 143 bp was detected in females, compared with two specific bands of 143 and 310 bp in males, indicating that the band patterns were clearly different between females and males. Since the set of primers also proved compatible with tissue and fecal samples, the results of the present study demonstrate that the present PCR could be a convenient tool for identifying the sex of leopard cats.
在本研究中，我们使用多重聚合酶链反应（PCR）来确定韩国豹猫（豹猫指名亚种）的性别。当我们使用基于ZFX和ZFY之间内含子区域的引物进行PCR时，在雌性中仅检测到一条143 bp的DNA条带，而在雄性中检测到143 bp和310 bp的两条特异性条带，这表明雌性和雄性的条带模式明显不同。由于该引物组也被证明与组织和粪便样本兼容，本研究结果表明，目前的PCR可作为鉴定豹猫性别的便捷工具。
