Ayyachamy M, Vatsala T M
Shri AMM Murugappa Chettiar Research Centre, Taramani, Chennai, India.
Lett Appl Microbiol. 2007 Nov;45(5):467-72. doi: 10.1111/j.1472-765X.2007.02223.x. Epub 2007 Sep 14.
To optimize the solid-state cultivation conditions for xylanase production using agriresidues and testing the biobleaching efficiency of xylanase on nonwoody plant fibre materials.
An extracellular cellulase free xylanase was produced from Bacillus subtilis C 01 using various inexpensive substrates under solid-state cultivation. High level of xylanase production (135 IU gds(-1)) was observed when grown on wheat bran followed by maize powder (50 IU gds(-1)). The maximum xylanase (136 IU gds(-1)) production was occurred in wheat bran-to-moisture ratio of 1 : 1 at 72 h. The xylanase pretreated pulp samples of banana, silk cotton and cotton showed an increased brightness of 19.6, 11.6 and 7.9%, respectively.
The enzyme-aided biobleaching results indicate that the xylanase has potential application in enhancing the brightness of nonwoody plant fibre pulp.
This is the first report on biobleaching of banana fibres, silk cotton and cotton pulps using xylanase. The biobleaching results of secondary fibres are promising and can be transferred to paper mills, which utilize nonwoody plant fibres as a raw material for paper production.
优化利用农业残余物生产木聚糖酶的固态培养条件，并测试木聚糖酶对非木本植物纤维材料的生物漂白效率。
在固态培养条件下，使用多种廉价底物从枯草芽孢杆菌C 01生产胞外无纤维素酶木聚糖酶。以麦麸为生长底物时，木聚糖酶产量较高（135 IU gds(-1)），其次是玉米粉（50 IU gds(-1)）。在72小时时，麦麸与水分比为1:1时木聚糖酶产量最高（136 IU gds(-1)）。经木聚糖酶预处理的香蕉、木棉和棉花纸浆样品的白度分别提高了19.6%、11.6%和7.9%。
酶辅助生物漂白结果表明，木聚糖酶在提高非木本植物纤维纸浆白度方面具有潜在应用价值。
这是关于使用木聚糖酶对香蕉纤维、木棉和棉花纸浆进行生物漂白的首次报道。二次纤维的生物漂白结果很有前景，可应用于以非木本植物纤维为造纸原料的造纸厂。
