一种用于牛C反应蛋白和血清淀粉样蛋白P成分的新纯化方法。
A new purification procedure for bovine C-reactive protein and serum amyloid P component.
作者信息
Sarikaputi M, Morimatsu M, Syuto B, Saito M, Naiki M
机构信息
Department of Veterinary Science, National Institute of Health, Tokyo, Japan.
出版信息
Int J Biochem. 1991;23(10):1137-42. doi: 10.1016/0020-711x(91)90155-g.
PMID:1786856
Abstract
- A new purification procedure was started with salting-out fractionation of serum proteins at 45-75% saturated ammonium sulfate concentration, followed by HE agarose affinity chromatography by which calcium-dependently bound CRP and SAP were purely eluted with EDTA-containing buffer. 2. Pure CRP and SAP were finally separated by DEAE-5PW HPLC. 3. This procedure gave recovery of 15 and 26%, and fold purification of 2650 and 2400 for CRP and SAP, respectively. 4. Each subunit of CRP and SAP had one intrasubunit disulfide bond, determined by reduction and carboxymethylation.
摘要
- 一种新的纯化方法开始于在45 - 75%饱和硫酸铵浓度下对血清蛋白进行盐析分级分离，随后进行HE琼脂糖亲和层析，通过该层析用含EDTA的缓冲液将钙依赖性结合的CRP和SAP纯洗脱。2. 纯的CRP和SAP最终通过DEAE - 5PW高效液相色谱法分离。3. 该方法对CRP和SAP的回收率分别为15%和26%，纯化倍数分别为2650和2400。4. 通过还原和羧甲基化测定，CRP和SAP的每个亚基都有一个亚基内二硫键。
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