Borges Adolfo, Rojas-Runjaic Fernando J M
Laboratorio de Toxinas Animales, Centro de Biociencias y Medicina Molecular, Instituto de Estudios Avanzados, Caracas, Venezuela.
Toxicon. 2007 Dec 1;50(7):1005-10. doi: 10.1016/j.toxicon.2007.07.019. Epub 2007 Aug 6.
An extensive field survey allowed us to expand the geographical distribution of the scorpion Tityus perijanensis in the Perijá range, western Zulia State, Venezuela, including areas where adult cases of severe scorpionism have been reported. 16S ribosomal RNA (rRNA) gene sequencing, DL(50) determination, and native PAGE suggest low genetic and venom proteomic divergence across the distribution range. The results also indicate phylogenetic divergence between T. perijanensis and T. discrepans, the species prevalent in northcentral Venezuela. T. perijanensis venom lethality (0.91-0.94 mg/kg) is comparable to that of the Brazilian T. serrulatus and ranks highest among toxic Venezuelan Tityus studied so far. The data indicate that the Perijá range should be included amongst the endemic areas of scorpionism of Venezuela and Colombia.
通过广泛的实地调查，我们得以扩大委内瑞拉苏利亚州西部佩里哈山脉中蝎子佩里哈肥尾蝎的地理分布范围，其中包括曾报告过严重蝎蜇伤成人病例的地区。16S核糖体RNA（rRNA）基因测序、半数致死剂量（DL[50]）测定以及非变性聚丙烯酰胺凝胶电泳（native PAGE）表明，在其分布范围内，该蝎子的遗传和毒液蛋白质组差异较小。结果还表明，佩里哈肥尾蝎与委内瑞拉中北部常见的差异肥尾蝎之间存在系统发育差异。佩里哈肥尾蝎的毒液致死率（0.91 - 0.94毫克/千克）与巴西锯齿肥尾蝎相当，在迄今研究的有毒委内瑞拉肥尾蝎中排名最高。数据表明，佩里哈山脉应被列入委内瑞拉和哥伦比亚的蝎蜇伤流行地区。
