Weyn Christine, Boulenouar Selma, Mathys Vanessa, Vanhoolandt Julie, Bernis Aurore, Fontaine Véronique
Laboratory of Molecular Virology, ISP/Institut Pasteur, rue Engeland 642, 1180 Brussels, Belgium.
J Virol Methods. 2007 Dec;146(1-2):405-8. doi: 10.1016/j.jviromet.2007.08.003. Epub 2007 Sep 14.
Human papillomavirus (HPV) types 45 and 51 are both considered as high risk types for the development of human cervical cancer. To optimize the detection of these two types in clinical samples, HPV-45 and HPV-51 specific primers were designed to amplify respectively a 141bp and a 266bp fragment from the L1 gene by polymerase chain reaction (PCR). The sensitivity and the specificity of these two PCR reactions were determined using varying amounts of HPV DNA containing plasmids and negative and positive controls. Overall, the sensitivity for the HPV-45 plasmid DNA is 10fg, while for HPV-51 the sensitivity is 1fg. This is equivalent to approximately 100 and 10 HPV genome copies per PCR reaction, respectively.
人乳头瘤病毒（HPV）45型和51型均被视为人类宫颈癌发生的高危型别。为优化临床样本中这两种型别的检测，设计了HPV - 45和HPV - 51特异性引物，通过聚合酶链反应（PCR）分别从L1基因扩增出141bp和266bp的片段。使用不同量含HPV DNA的质粒以及阴性和阳性对照来确定这两种PCR反应的敏感性和特异性。总体而言，HPV - 45质粒DNA的敏感性为10fg，而HPV - 51的敏感性为1fg。这分别相当于每个PCR反应约100个和10个HPV基因组拷贝。
