Chollet C, Chanseau C, Brouillaud B, Durrieu M C
INSERM U577, Bordeaux, F-33076, France.
Biomol Eng. 2007 Nov;24(5):477-82. doi: 10.1016/j.bioeng.2007.07.012. Epub 2007 Aug 7.
The aim of this study was to graft RGD peptides with well controlled densities onto poly(ethylene terephthalate) (PET) film surfaces. Biomimetic modifications were performed by means of a four-step reaction procedure: surface modification in order to create -COOH groups onto polymer surface, coupling agent grafting and finally immobilization of peptides. The originality of this work is to evaluate several grafted densities peptides. Toluidine blue and high-resolution mu-imager (using [(3)H]-Lys) were used to evaluate densities. Moreover, mu-imager has exhibited the stability of peptides grafted onto the surface when treated under harsh conditions. Benefits of the as-proposed method were related to the different concentrations of peptides grafted onto the surface as well as the capacity of RGD peptide to interact with integrin receptors.
本研究的目的是将具有可控密度的RGD肽接枝到聚对苯二甲酸乙二酯（PET）薄膜表面。通过四步反应程序进行仿生修饰：表面改性以在聚合物表面产生-COOH基团，接枝偶联剂，最后固定肽。这项工作的独特之处在于评估几种接枝密度的肽。使用甲苯胺蓝和高分辨率μ成像仪（使用[(3)H]-Lys）来评估密度。此外，μ成像仪显示了在苛刻条件下处理时接枝到表面的肽的稳定性。所提出方法的优点与接枝到表面的不同浓度的肽以及RGD肽与整合素受体相互作用的能力有关。
