Zhao Qiang, Wan Changxiu, Liu Jianwei, Qiu Kai
School of Pharmacy, Sichuan University, Chengdu 610041.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2003 Sep;20(3):384-7.
The aim of this experiment is to graft synthesizing Arg-Gly-Asp peptides (RGD) on the surface of polymer materials, combine endothelial cells with its special site, enhance the adhesion of endothelial cells on the surface, promote the blood compatibility of the biomaterials. Carboxy group (-COOH) was grafted on the materials surface by ultraviolet (UV) radiation, and the RGD serial obtained by liquid phase synthesis was successfully grafted on the disposed materials by chemical reaction. The endothelialization experiment was made also. The grafting results were measured by X-ray photoelectron spectroscopy (XPS), and endothelialization was observed using optical microscope and scanning electron microscope (SEM). The results indicated that the method improves the effect of materials endothelialization. The experiment has made successful use of UV grafting and chemical coupling methods to graft bioactive RGD onto PET film surface. This is a new method of grafting bioactive peptide.
本实验的目的是将合成的精氨酸-甘氨酸-天冬氨酸肽（RGD）接枝到高分子材料表面，使其特殊位点与内皮细胞结合，增强内皮细胞在材料表面的黏附，提高生物材料的血液相容性。通过紫外线（UV）辐射在材料表面接枝羧基（-COOH），然后通过化学反应将液相合成得到的RGD系列成功接枝到处理过的材料上。同时进行了内皮化实验。采用X射线光电子能谱（XPS）测定接枝结果，利用光学显微镜和扫描电子显微镜（SEM）观察内皮化情况。结果表明，该方法提高了材料内皮化效果。本实验成功利用UV接枝和化学偶联方法将生物活性RGD接枝到PET薄膜表面。这是一种接枝生物活性肽的新方法。
