Al-Salehi S K, Wood D J, Hatton P V
Centre for Biomaterials & Tissue Engineering, Department of Adult Dental Care, School of Clinical Dentistry, University of Sheffield, Claremont Crescent, Sheffield S10 2TA, UK.
J Dent. 2007 Nov;35(11):845-50. doi: 10.1016/j.jdent.2007.08.001. Epub 2007 Sep 14.
Tooth bleaching agents may adversely affect tooth structure. The aim of this study was to investigate the effect of hydrogen peroxide concentration on mineral loss and microhardness of bovine teeth.
Twenty-six freshly extracted intact bovine incisor teeth were stored in distilled water. Five teeth were sectioned and four samples (2 mm x 2 mm x 1.5 mm) each of enamel and dentine were obtained from each tooth. The samples of enamel and dentine were divided into four groups and immersed in either 0%, 3%, 10% or 30% (w/v) hydrogen peroxide solutions for 24h at 37 degrees C. Samples from the solutions were taken for ion release analysis using inductively coupled plasma mass spectrometry. The remaining 21 teeth were mounted in epoxy resin and the upper surface of the specimens were ground and polished to expose the enamel and dentine for microhardness measurements. These specimens were randomly divided into three equal groups and Vickers microhardness values were recorded on the enamel and dentine surfaces of each group before and after bleaching.
The differences in ion release concentration after treatment with 0% (control) and each of 3%, 10% and 30% hydrogen peroxide (w/v) were statistically significant (p<0.025). The release of calcium and phosphorous ions increased with increasing hydrogen peroxide concentrations. A significant reduction (p<0.05) in Vickers microhardness values for enamel was recorded after bleaching.
Ion release from both enamel and dentine increased with increasing hydrogen peroxide concentration. Microhardness of enamel decreased significantly with bleaching.
牙齿漂白剂可能会对牙齿结构产生不利影响。本研究的目的是调查过氧化氢浓度对牛牙矿物质流失和显微硬度的影响。
26颗新鲜拔除的完整牛切牙保存在蒸馏水中。将5颗牙齿切片，从每颗牙齿中获取釉质和牙本质的四个样本（2毫米×2毫米×1.5毫米）。将釉质和牙本质样本分为四组，在37摄氏度下分别浸泡在0%、3%、10%或30%（w/v）的过氧化氢溶液中24小时。从溶液中取出样本，使用电感耦合等离子体质谱法进行离子释放分析。将其余21颗牙齿安装在环氧树脂中，对标本的上表面进行研磨和抛光，以暴露釉质和牙本质进行显微硬度测量。将这些标本随机分为三组，记录每组在漂白前后釉质和牙本质表面的维氏显微硬度值。
用0%（对照）以及3%、10%和30%过氧化氢（w/v）处理后，离子释放浓度的差异具有统计学意义（p<0.025）。钙和磷离子的释放随着过氧化氢浓度的增加而增加。漂白后，釉质的维氏显微硬度值显著降低（p<0.05）。
随着过氧化氢浓度的增加，釉质和牙本质的离子释放均增加。漂白后，釉质的显微硬度显著降低。
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