Maung N L, Wohland T, Hsu C-Y S
Department of Preventive Dentistry, Faculty of Dentistry, 5 Lower Kent Ridge Road, National University of Singapore, Singapore 119074.
J Dent. 2007 Oct;35(10):794-9. doi: 10.1016/j.jdent.2007.07.012. Epub 2007 Sep 14.
Organic matrix (OM) has been hypothesized as a key player in the laser-induced retardation of enamel diffusion (LRED).
Therefore, this study was aimed to quantify the contribution of OM in LRED.
Four groups of enamel sections (n=10) were assigned to 'normal', 'laser treated', 'OM extracted' and 'laser+OM extraction' groups for measurement of diffusion coefficient (DC) using fluorescence recovery after photobleaching (FRAP) and fluorophores transport study (FTS). Er:YAG laser treatment and OM extraction were performed on respective groups. Sections were characterized with stereomicroscopy and polarized light microscopy. Treatment effects were statistically assessed with a factorial ANOVA.
DC measured by FRAP and FTS coupled with confocal microscopy revealed the significant effect of OM (p=0.001) and laser treatment (p<0.01). After OM extraction, the laser effect on diffusion decreased about 34-75%, confirming the significant role of OM in LRED.
Both FRAP and FTS may be promising tools to quantify enamel DC.
有机基质（OM）被假定为激光诱导牙釉质扩散延迟（LRED）的关键因素。
因此，本研究旨在量化OM在LRED中的作用。
将四组牙釉质切片（n = 10）分为“正常”、“激光处理”、“OM提取”和“激光+OM提取”组，使用光漂白后荧光恢复（FRAP）和荧光团转运研究（FTS）测量扩散系数（DC）。对各相应组进行铒钇铝石榴石激光处理和OM提取。切片用体视显微镜和偏光显微镜进行表征。治疗效果采用析因方差分析进行统计学评估。
通过FRAP和FTS结合共聚焦显微镜测量的DC显示了OM（p = 0.001）和激光处理（p < 0.01）的显著影响。OM提取后，激光对扩散的影响降低了约34 - 75%，证实了OM在LRED中的重要作用。
FRAP和FTS都可能是量化牙釉质DC的有前景的工具。
