铒钇铝石榴石激光调制的牙釉质扩散（第2部分）。有机基质。

Enamel diffusion modulated by Er:YAG laser (Part 2). Organic matrix.

作者信息

Maung N L, Wohland T, Hsu C-Y S

机构信息

Department of Preventive Dentistry, Faculty of Dentistry, 5 Lower Kent Ridge Road, National University of Singapore, Singapore 119074.

出版信息

J Dent. 2007 Oct;35(10):794-9. doi: 10.1016/j.jdent.2007.07.012. Epub 2007 Sep 14.

DOI:10.1016/j.jdent.2007.07.012

PMID:17869404

Abstract

UNLABELLED

Organic matrix (OM) has been hypothesized as a key player in the laser-induced retardation of enamel diffusion (LRED).

OBJECTIVES

Therefore, this study was aimed to quantify the contribution of OM in LRED.

METHODS

Four groups of enamel sections (n=10) were assigned to 'normal', 'laser treated', 'OM extracted' and 'laser+OM extraction' groups for measurement of diffusion coefficient (DC) using fluorescence recovery after photobleaching (FRAP) and fluorophores transport study (FTS). Er:YAG laser treatment and OM extraction were performed on respective groups. Sections were characterized with stereomicroscopy and polarized light microscopy. Treatment effects were statistically assessed with a factorial ANOVA.

RESULTS

DC measured by FRAP and FTS coupled with confocal microscopy revealed the significant effect of OM (p=0.001) and laser treatment (p<0.01). After OM extraction, the laser effect on diffusion decreased about 34-75%, confirming the significant role of OM in LRED.

CONCLUSION

Both FRAP and FTS may be promising tools to quantify enamel DC.

摘要

未标记

有机基质（OM）被假定为激光诱导牙釉质扩散延迟（LRED）的关键因素。

目的

因此，本研究旨在量化OM在LRED中的作用。

方法

将四组牙釉质切片（n = 10）分为“正常”、“激光处理”、“OM提取”和“激光+OM提取”组，使用光漂白后荧光恢复（FRAP）和荧光团转运研究（FTS）测量扩散系数（DC）。对各相应组进行铒钇铝石榴石激光处理和OM提取。切片用体视显微镜和偏光显微镜进行表征。治疗效果采用析因方差分析进行统计学评估。