[三七皂苷R1体外诱导HL-60细胞系分化的初步研究]

[Preliminary studies of notoginsenoside R1-induced differentiation of HL-60 cell lines in vitro].

作者信息

Xu L, Wang B, Gao J

机构信息

Department of Pathophysiology.

出版信息

Hua Xi Yi Ke Da Xue Xue Bao. 1991 Jun;22(2):124-7.

PMID:1786946

Abstract

Our experiments have proved that human promyelocytic leukemia cells (HL-60) could be induced to differentiate in vitro by notoginsenoside R1 into mature neutrophils. The morphological results showed that 68% of HL-60 cells were differentiated in 80 microgram/ml inducer for 5 days, in which metamyelocytes were 32%, banded neutrophils 30% and segmented neutrophils 6% 3H-TdR and 3H-UR incorporate assay showed that notoginsenoside R1 effected synthesis of DNA and RNA while it induced differentiation of HL-60 cell. At 48 h, percent of incorporate inhibition was 26.32% with 3H-TdR and 18.57% with 3H-UR; at 72 h, 17.46% and 21.76%, respectively.

摘要

我们的实验已经证明，三七总皂苷R1可在体外诱导人早幼粒细胞白血病细胞（HL-60）分化为成熟的中性粒细胞。形态学结果显示，在80微克/毫升诱导剂作用5天后，68%的HL-60细胞发生分化，其中晚幼粒细胞占32%，带状核中性粒细胞占30%，分叶核中性粒细胞占6%。3H-胸腺嘧啶脱氧核苷（3H-TdR）和3H-尿嘧啶核苷（3H-UR）掺入试验表明，三七总皂苷R1在诱导HL-60细胞分化的同时影响DNA和RNA的合成。在48小时时，3H-TdR的掺入抑制率为26.32%，3H-UR的掺入抑制率为18.57%；在72小时时，分别为17.46%和21.76%。