Chang Microsugar, Wang Jhih-Hao, Lee Han-Jung
Department of Life Science, National Dong Hwa University, Hualien 97401, Taiwan.
J Biochem Biophys Methods. 2008 Apr 24;70(6):1199-202. doi: 10.1016/j.jbbm.2007.08.002. Epub 2007 Aug 24.
DNA molecular weight markers are routinely used in agarose gel electrophoresis. Here we report a method called PCR-synthesized marker (PSM) to generate DNA molecular rulers by PCR in the laboratory. This strategy can also be used to produce 100 bp RNA molecular weight markers by run-off transcription.
DNA分子量标准物常用于琼脂糖凝胶电泳。在此，我们报告一种名为PCR合成标准物（PSM）的方法，可在实验室通过PCR生成DNA分子尺。该策略也可用于通过连续转录产生100bp的RNA分子量标准物。
