Kudo M, Vera D R, Trudeau W L, Stadalnik R C
Division of Nuclear Medicine, University of California, Davis, Medical Center, Sacramento 95817.
Int J Rad Appl Instrum B. 1991;18(7):663-6. doi: 10.1016/0883-2897(91)90003-4.
[Technetium-99m]galactosyl-neoglycoalbumin (TcNGA) is a synthetic radioligand specific to the receptor, hepatic binding protein (HBP), which resides exclusively at the cell surface of hepatocytes. If the TcNGA time-activity data are to provide valid estimates of receptor biochemistry via pharmacokinetic modeling, the shape of the hepatic uptake curve must change with alterations in receptor concentration or affinity. An index of TcNGA hepatic uptake, T90, was obtained by calculating the time at which the liver time-activity curve reached 90% of its maximum. Receptor quantity was measured by Scatchard binding assay of liver biopsy samples obtained prior to each TcNGA study. The range of T90 was 3.7-16.8 min; HBP quantity ranged from 0.022 to 0.100 mumol. Significant correlation (r) and slope (b) was observed between T90 and HBP quantity (r = 0.82, P = 0.003; tb = 3.98, P = 0.003). We concluded that the hepatic uptake of TcNGA is sensitive to HBP quantity.
[锝-99m]半乳糖基新糖白蛋白（TcNGA）是一种对肝结合蛋白（HBP）受体具有特异性的合成放射性配体，HBP仅存在于肝细胞的细胞表面。如果TcNGA时间-活性数据要通过药代动力学建模提供受体生物化学的有效估计值，肝摄取曲线的形状必须随受体浓度或亲和力的改变而变化。通过计算肝脏时间-活性曲线达到其最大值的90%时的时间，获得了TcNGA肝摄取指数T90。在每次TcNGA研究之前，通过对肝活检样本进行Scatchard结合测定来测量受体数量。T90的范围为3.7至16.8分钟；HBP数量范围为0.022至0.100μmol。观察到T90与HBP数量之间存在显著相关性（r）和斜率（b）（r = 0.82，P = 0.003；tb = 3.98，P = 0.003）。我们得出结论，TcNGA的肝摄取对HBP数量敏感。
