Langmuir V K, McGann J K, Buchegger F, Sutherland R M
University of Rochester Cancer Center, NY 14642.
Int J Rad Appl Instrum B. 1991;18(7):753-64. doi: 10.1016/0883-2897(91)90014-c.
Intact IgG1 and F(ab')2 anti-carcinoembryonic antigen antibodies penetrate human colon adenocarcinoma multicell spheroids much more slowly than Fab fragments and the molecular weight and the binding site valency appear to be the most important factor in determining the rate of penetration. The rate is also influenced considerably by the number of antigen binding sites per cell, with a high antigen concentration slowing penetration appreciably. The tumor cell architecture appears to have a minor effect on antibody penetration when compared to antibody size or antigen concentration.
完整的IgG1和F(ab')2抗癌胚抗原抗体穿透人结肠腺癌多细胞球体的速度比Fab片段慢得多，分子量和结合位点价态似乎是决定穿透速率的最重要因素。该速率还受到每个细胞抗原结合位点数量的显著影响，高抗原浓度会明显减缓穿透速度。与抗体大小或抗原浓度相比，肿瘤细胞结构对抗体穿透的影响似乎较小。
