三种近交系小鼠体外脑乙酰胆碱酯酶对久效磷的反应

In vitro brain acetylcholinesterase response among three inbred strains of mice to monocrotophos.

作者信息

Rao J V, Swamy A N, Yamin S

机构信息

Biology Division, Indian Institute of Chemical Technology, Hyderabad.

出版信息

J Environ Sci Health B. 1991 Aug;26(4):449-58. doi: 10.1080/03601239109372748.

DOI:10.1080/03601239109372748

PMID:1787264

Abstract

The strain differences in the neurotoxic potential of monocrotophos (MCP) were assessed by determining the inhibition of brain acetylcholinesterase (AChE) in BALB/cAnN, DBA/2J and C57BL/6J in vitro. MCP being a competitive inhibitor for AChE, alters the Km values widely among these inbred strains. Comparatively least alterations in Km were found in BALB/cAnN and maximum in DBA/2J. Based on the Ki values DBA/2J was found to be the most sensitive strain to MCP inhibition followed by C57BL/6J and BALB/cAnN.

摘要

通过在体外测定久效磷（MCP）对BALB/cAnN、DBA/2J和C57BL/6J小鼠脑乙酰胆碱酯酶（AChE）的抑制作用，评估了久效磷神经毒性潜力的品系差异。久效磷作为AChE的竞争性抑制剂，在这些近交系中会广泛改变Km值。在BALB/cAnN中发现Km的变化相对最小，而在DBA/2J中变化最大。基于Ki值，发现DBA/2J是对久效磷抑制最敏感的品系，其次是C57BL/6J和BALB/cAnN。