Konar Vahit, Kara Haki, Yilmaz Mahmut, Dayangac Alpaslan, Karatas Fikret
Department of Biology, College of Science, Firat University, Elazig, Turkey.
Biol Trace Elem Res. 2007 Aug;118(2):131-7. doi: 10.1007/s12011-007-0009-9.
The present study was carried to evaluate the protective effects of melatonin alone and vitamin E with selenium combination against high dose cadmium-induced oxidative stress in rats. The control group received subcutaneous physiological saline. The first study group administered cadmium chloride (CdCl2) by subcutaneous injection of dose of 1 mg/kg. The second study group administered cadmium plus vitamin E with selenium (1 mg/kg sodium selenite with 60 mg/kg vitamin E); the third study group administered cadmium plus 10 mg/kg melatonin (MLT); the fourth study group administered CdCl2 plus a combination of melatonin in addition to vitamin E and selenium for a month. Determination levels of plasma malondialdehyde (MDA), glutathione peroxidase (GSH-Px), blood superoxide dismutase (SOD), creatinine alanine transaminase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), blood urea nitrogen (BUN), and urea were measured in serum. In only CdCl2 administered group, the MDA, creatinine, ALT, AST, ALP, and urea levels in the serum were significantly higher than the control group (p < 0.05). Whereas in all other groups, this values were significantly lower than the only CdCl2 administered group (p < 0.05). Erythrocytes GSH-Px, serum SOD activities of only CdCl2 received group were significantly lower than the control group (p < 0.05). In conclusion, vitamin E + Se, melatonin and vitamin E, and Se, in addition to MLT combinations, had protective effects against high dose cadmium-induced oxidative damage.
本研究旨在评估褪黑素单独使用以及维生素E与硒联合使用对高剂量镉诱导的大鼠氧化应激的保护作用。对照组皮下注射生理盐水。第一研究组皮下注射剂量为1mg/kg的氯化镉（CdCl2）。第二研究组给予镉加维生素E与硒（1mg/kg亚硒酸钠加60mg/kg维生素E）；第三研究组给予镉加10mg/kg褪黑素（MLT）；第四研究组给予CdCl2加褪黑素以及维生素E和硒的组合，持续一个月。测定血清中血浆丙二醛（MDA）、谷胱甘肽过氧化物酶（GSH-Px）、血液超氧化物歧化酶（SOD）、肌酐丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）、碱性磷酸酶（ALP）、血尿素氮（BUN）和尿素的水平。仅给予CdCl2的组中，血清中的MDA、肌酐、ALT、AST、ALP和尿素水平显著高于对照组（p<0.05）。而在所有其他组中，这些值显著低于仅给予CdCl2的组（p<0.05）。仅给予CdCl2组的红细胞GSH-Px、血清SOD活性显著低于对照组（p<0.05）。总之，维生素E+硒、褪黑素以及维生素E和硒，再加上MLT组合，对高剂量镉诱导的氧化损伤具有保护作用。
