Lee William, Tillo Desiree, Bray Nicolas, Morse Randall H, Davis Ronald W, Hughes Timothy R, Nislow Corey
Department of Genetics, Stanford University School of Medicine, Stanford, California 94305-5120, USA.
Nat Genet. 2007 Oct;39(10):1235-44. doi: 10.1038/ng2117. Epub 2007 Sep 16.
We present the first complete high-resolution map of nucleosome occupancy across the whole Saccharomyces cerevisiae genome, identifying over 70,000 positioned nucleosomes occupying 81% of the genome. On a genome-wide scale, the persistent nucleosome-depleted region identified previously in a subset of genes demarcates the transcription start site. Both nucleosome occupancy signatures and overall occupancy correlate with transcript abundance and transcription rate. In addition, functionally related genes can be clustered on the basis of the nucleosome occupancy patterns observed at their promoters. A quantitative model of nucleosome occupancy indicates that DNA structural features may account for much of the global nucleosome occupancy.
我们展示了首张完整的酿酒酵母全基因组高分辨率核小体占位图谱，确定了超过70000个定位核小体，占据了基因组的81%。在全基因组范围内，先前在一部分基因中鉴定出的持续核小体缺失区域划定了转录起始位点。核小体占位特征和总体占位都与转录本丰度和转录速率相关。此外，功能相关的基因可以根据其启动子处观察到的核小体占位模式进行聚类。核小体占位的定量模型表明，DNA结构特征可能在很大程度上解释了全局核小体占位情况。
