MegaPlex聚合酶链式反应：一种多重扩增策略。

MegaPlex PCR: a strategy for multiplex amplification.

作者信息

Meuzelaar Linda Strömqvist, Lancaster Owen, Pasche J Paul, Kopal Guido, Brookes Anthony J

机构信息

Department of Genetics, University of Leicester, University Road, Leicester, LE1 7RH, UK.

出版信息

Nat Methods. 2007 Oct;4(10):835-7. doi: 10.1038/nmeth1091. Epub 2007 Sep 16.

DOI:10.1038/nmeth1091

PMID:17873887

Abstract

'MegaPlex PCR' is a robust technology for highly multiplexed amplification of specific DNA sequences. It uses target-specific pairs of PCR primers that are physically separated by surface immobilization. Initial surface-based amplification cycles are then coupled to efficient solution-phase PCR using one common primer pair. We demonstrate this method by co-amplifying and genotyping 75 unselected human single-nucleotide polymorphism (SNP) loci.

摘要

“MegaPlex聚合酶链反应”是一种用于高度多重扩增特定DNA序列的强大技术。它使用通过表面固定物理分离的针对靶标的聚合酶链反应引物对。然后，基于表面的初始扩增循环与使用一个通用引物对的高效溶液相聚合酶链反应相结合。我们通过对75个未选择的人类单核苷酸多态性（SNP）位点进行共扩增和基因分型来证明这种方法。