Bonney R C, Beesley J S, Franks S
Department of Chemical Pathology, St Mary's Hospital Medical School, London, UK.
J Reprod Fertil. 1991 Nov;93(2):449-60. doi: 10.1530/jrf.0.0930449.
Primary cultures of endometrial glands and stromal cells were labelled with [14C]-arachidonic acid for 4 h before exposure to either the calcium ionophore, A23187 (which activates phospholipase A2 (PLA2) by increasing intracellular calcium concentrations) or sodium fluoride (which activates a G-protein). Calcium ionophore (0.5-50 mumol/l) stimulated a dose- and time-dependent release of arachidonic acid from endometrial glands. Incubation with ionophore (10 mumol/l) for 1 h released 22% of the incorporated arachidonic acid. There was a corresponding decrease in phospholipids and no loss from triglycerides. Stromal cells were unresponsive to ionophore. Fluoride (10 mmol/l) stimulated a release of arachidonic acid from stromal cells and endometrial glands (6.5% of the total arachidonic acid incorporated). In stromal cells, arachidonic acid was released from triglycerides in Day-1 cultures and from phospholipids in Day-2 cultures. In both Day-1 and Day-2 cultures of endometrial glands, arachidonic acid was released from phospholipids, but not from triglycerides. Among the phospholipids, phosphatidylcholine was always the major source of arachidonic acid. Arachidonic acid release from endometrial glands and stromal cells may be mediated by activation of PLA2 (or phospholipase C) via a G-protein, but in glands calcium ionophore may have a direct effect on PLA2. The response to calcium ionophore may reflect the differences in calcium requirements of the two endometrial PLA2 isoenzymes.
在暴露于钙离子载体A23187（通过增加细胞内钙离子浓度激活磷脂酶A2（PLA2））或氟化钠（激活G蛋白）之前，用[14C] - 花生四烯酸对子宫内膜腺体和基质细胞的原代培养物进行标记4小时。钙离子载体（0.5 - 50μmol / l）刺激子宫内膜腺体中花生四烯酸呈剂量和时间依赖性释放。用离子载体（10μmol / l）孵育1小时释放了22％掺入的花生四烯酸。磷脂相应减少，甘油三酯无损失。基质细胞对离子载体无反应。氟化物（10 mmol / l）刺激基质细胞和子宫内膜腺体释放花生四烯酸（占掺入的总花生四烯酸的6.5％）。在基质细胞中，花生四烯酸在第1天培养物中从甘油三酯释放，在第2天培养物中从磷脂释放。在子宫内膜腺体的第1天和第2天培养物中，花生四烯酸均从磷脂释放，但不从甘油三酯释放。在磷脂中，磷脂酰胆碱始终是花生四烯酸的主要来源。子宫内膜腺体和基质细胞中花生四烯酸的释放可能通过G蛋白激活PLA2（或磷脂酶C）介导，但在腺体中钙离子载体可能对PLA2有直接作用。对钙离子载体的反应可能反映了两种子宫内膜PLA2同工酶对钙需求的差异。
