冷冻解冻后的射出精子对体外成熟培养的猪卵母细胞的体外穿透能力。

In-vitro penetration of pig oocytes matured in culture by frozen-thawed ejaculated spermatozoa.

作者信息

Wang W H, Niwa K, Okuda K

机构信息

Division of Animal Science, Faculty of Agriculture, Okayama University, Japan.

出版信息

J Reprod Fertil. 1991 Nov;93(2):491-6. doi: 10.1530/jrf.0.0930491.

DOI:10.1530/jrf.0.0930491

PMID:1787470

Abstract

Pig oocytes matured in culture were inseminated with frozen-thawed ejaculated spermatozoa without preincubation in modified tissue culture medium (TCM) 199. High penetration rates (85-89%) and increased incidence of polyspermy were obtained at 25-100 x 10(6) spermatozoa/ml. Wide variation in penetration rates (16-89%) was observed in oocytes inseminated in medium containing 5mM caffeine and at 25-50 x 10(6) spermatozoa/ml obtained from 6 boars, regardless of sperm motility. At 25-50 x 10(6) spermatozoa/ml, penetration rates of oocytes were dependent upon the concentration of caffeine in the medium: there was no penetration without caffeine, but penetration was highest (89%) with 5mM caffeine. None of the oocytes was penetrated in the medium supplemented with heparin at 5-40 micrograms/ml. When heparin was included in the medium with 5mM caffeine, it inhibited the efficacy of caffeine to promote sperm penetration of oocytes.

摘要

将在体外成熟的猪卵母细胞与经冷冻解冻的射出精子进行受精，精子无需在改良的组织培养液（TCM）199中预孵育。当精子浓度为25 - 100×10⁶/ml时，可获得较高的穿透率（85 - 89%），但多精入卵的发生率也有所增加。在含有5mM咖啡因的培养液中，当精子浓度为25 - 50×10⁶/ml且取自6头公猪时，无论精子活力如何，卵母细胞的穿透率都有很大差异（16 - 89%）。当精子浓度为25 - 50×10⁶/ml时，卵母细胞的穿透率取决于培养液中咖啡因的浓度：无咖啡因时无穿透发生，但在含5mM咖啡因时穿透率最高（89%）。在添加了5 - 40μg/ml肝素的培养液中，没有卵母细胞被穿透。当在含5mM咖啡因的培养液中加入肝素时，它会抑制咖啡因促进精子穿透卵母细胞的效果。