利用转基因猪的冷冻附睾精子，通过体外受精和输卵管内授精技术生产糖尿病后代。
Production of diabetic offspring using cryopreserved epididymal sperm by in vitro fertilization and intrafallopian insemination techniques in transgenic pigs.
作者信息
Umeyama Kazuhiro, Honda Kasumi, Matsunari Hitomi, Nakano Kazuaki, Hidaka Tatsuro, Sekiguchi Keito, Mochizuki Hironori, Takeuchi Yasuhiro, Fujiwara Tsukasa, Watanabe Masahito, Nagaya Masaki, Nagashima Hiroshi
机构信息
Laboratory of Developmental Engineering, Department of Life Sciences, School of Agriculture, Meiji University, Kanagawa 214-8571, Japan.
出版信息
J Reprod Dev. 2013 Dec 17;59(6):599-603. doi: 10.1262/jrd.2013-069. Epub 2013 Aug 24.
Abstract
Somatic cell nuclear transfer (SCNT) is a useful technique for creating pig strains that model human diseases. However, production of numerous cloned disease model pigs by SCNT for large-scale experiments is impractical due to its complexity and inefficiency. In the present study, we aimed to establish an efficient procedure for proliferating the diabetes model pig carrying the mutant human hepatocyte nuclear factor-1α gene. A founder diabetes transgenic cloned pig was generated by SCNT and treated with insulin to allow for normal growth to maturity, at which point epididymal sperm could be collected for cryopreservation. In vitro fertilization and intrafallopian insemination using the cryopreserved epididymal sperm resulted in diabetes model transgenic offspring. These results suggest that artificial reproductive technology using cryopreserved epididymal sperm could be a practical option for proliferation of genetically modified disease model pigs.
摘要
体细胞核移植（SCNT）是创建模拟人类疾病的猪品系的一种有用技术。然而，由于其复杂性和低效率，通过SCNT生产大量克隆疾病模型猪用于大规模实验是不切实际的。在本研究中，我们旨在建立一种有效的程序来增殖携带突变型人类肝细胞核因子-1α基因的糖尿病模型猪。通过SCNT产生了一头奠基性糖尿病转基因克隆猪，并用胰岛素进行处理以使其正常生长至成熟，此时可收集附睾精子进行冷冻保存。使用冷冻保存的附睾精子进行体外受精和输卵管内授精产生了糖尿病模型转基因后代。这些结果表明，使用冷冻保存的附睾精子的人工生殖技术可能是增殖转基因疾病模型猪的一种实用选择。
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