Kim Jeong Ah, Jeon Un Sil, Kwon Min Seong, Lim Sun Woo, Kwon H Moo
Department of Medicine, University of Maryland, Baltimore, Maryland, USA.
Methods Enzymol. 2007;428:253-67. doi: 10.1016/S0076-6879(07)28014-0.
The TonE-binding protein (TonEBP) is a transcriptional activator in the Rel family that includes NFkappaB and NFAT. TonEBP is critical for the development and function of the renal medulla, which is a major regulator of water homeostasis. TonEBP is also implicated in diabetic nephropathy and inflammation. Established methods for biochemical and histochemical detection and functional analysis of TonEBP, including identification of novel TonEBP target genes, are described for those who are interested in investigating function and regulation of TonEBP.
张力应答元件结合蛋白（TonEBP）是Rel家族中的一种转录激活因子，该家族包括核因子κB（NFκB）和活化T细胞核因子（NFAT）。TonEBP对肾髓质的发育和功能至关重要，而肾髓质是水稳态的主要调节者。TonEBP还与糖尿病肾病和炎症有关。本文为那些有兴趣研究TonEBP功能和调控的人描述了用于TonEBP生化和组织化学检测及功能分析的既定方法，包括鉴定新的TonEBP靶基因。
