Guntinas-Lichius O, Angelov D N
Klinik und Poliklinik für Hals-, Nasen-, Ohrenkranke, Friedrich-Schiller-Universität, Lessingstrasse 2, 07740 Jena.
HNO. 2008 Feb;56(2):122-30. doi: 10.1007/s00106-007-1610-0.
Using a combination of the following, it is possible to investigate procedures to improve the morphological and functional regeneration of the facial nerve in animal models: 1) retrograde fluorescence tracing to analyse collateral axonal sprouting and the selectivity of reinnervation of the mimic musculature, 2) immunohistochemistry to analyse both the terminal axonal sprouting in the muscles and the axon reaction within the nucleus of the facial nerve, the peripheral nerve, and its environment, and 3) digital motion analysis of the muscles. To obtain good functional facial nerve regeneration, a reduction of terminal sprouting in the mimic musculature seems to be more important than a reduction of collateral sprouting at the lesion site. Promising strategies include acceleration of nerve regeneration, forced induced use of the paralysed face, mechanical stimulation of the face, and transplantation of nerve-growth-promoting olfactory epithelium at the lesion site.
通过结合以下方法，可以在动物模型中研究改善面神经形态和功能再生的程序：1）逆行荧光追踪，以分析侧支轴突发芽和表情肌再支配的选择性；2）免疫组织化学，以分析肌肉中的终末轴突发芽以及面神经核、周围神经及其周围环境中的轴突反应；3）肌肉的数字运动分析。为了获得良好的面神经功能再生，减少表情肌中的终末芽生似乎比减少损伤部位的侧支芽生更为重要。有前景的策略包括加速神经再生、强制使用麻痹的面部、对面部进行机械刺激以及在损伤部位移植促进神经生长的嗅上皮。
