多穗罗勒（Basilicum polystachyon (L) Moench.）叶提取物的抗菌活性

Antimicrobial activity of leaf extract of Basilicum polystachyon (L) Moench.

作者信息

Chakraborty D, Mandal S M, Chakraborty J, Bhattacharyaa P K, Bandyopadhyay A, Mitra A, Gupta K

机构信息

Department of Botany, The University of Burdwan, Burdwan 713 104, India.

出版信息

Indian J Exp Biol. 2007 Aug;45(8):744-8.

PMID:17877153

Abstract

Phenolic extract of leaves of Basilicum polystachyon (L) Moench was tested for in vitro antimicrobial activity against five bacteria (Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus subtilis, Micrococcus leuteus) and three fungi (Fusarium oxysporum, Aspergillus niger, Helminthosporium oryzae). Efficacy of organic solvents, methanol and ethanol, as agents for extraction was compared with acidic water (2M; HCl). High-pressure liquid chromatographic (HPLC) data showed that acidic extraction (2M; HCl) resulted in higher yield of caffeic acid (0.437 mg g(-1)) and rosmarinic acid (0.919 mg g(-1)). Acidic extract showed high activity against Gram (+) ve bacteria, but was less active against Gram (-) ve bacteria. Amongst the tested fungi, maximum activity was exhibited against Aspergillus niger. This is the first report on the phenolic constituents and bioactivity of B. polystachyon.

摘要

对多穗罗勒（Basilicum polystachyon (L) Moench）叶片的酚类提取物进行了体外抗菌活性测试，测试对象包括五种细菌（大肠杆菌、铜绿假单胞菌、金黄色葡萄球菌、枯草芽孢杆菌、藤黄微球菌）和三种真菌（尖孢镰刀菌、黑曲霉、稻瘟病菌）。将有机溶剂甲醇和乙醇作为提取剂的效果与酸性水（2M；HCl）进行了比较。高压液相色谱（HPLC）数据表明，酸性提取（2M；HCl）得到的咖啡酸（0.437 mg g(-1)）和迷迭香酸（0.919 mg g(-1)）产量更高。酸性提取物对革兰氏阳性菌表现出高活性，但对革兰氏阴性菌活性较低。在所测试的真菌中，对黑曲霉的活性最高。这是关于多穗罗勒酚类成分和生物活性的首次报道。