四种喀麦隆食用植物对表达外排泵的多重耐药革兰氏阴性菌的抗生素增效活性。
Antibiotic-potentiation activities of four Cameroonian dietary plants against multidrug-resistant Gram-negative bacteria expressing efflux pumps.
机构信息
Department of Biochemistry, Faculty of Science, University of Dschang, P,O, Box 67, Dschang, Cameroon.
出版信息
BMC Complement Altern Med. 2014 Jul 21;14:258. doi: 10.1186/1472-6882-14-258.
BACKGROUND
The continuous spread of multidrug-resistant (MDR) bacteria, partially due to efflux pumps drastically reduced the efficacy of the antibiotic armory, increasing the frequency of therapeutic failure. The search for new compounds to potentiate the efficacy of commonly used antibiotics is therefore important. The present study was designed to evaluate the ability of the methanol extracts of four Cameroonian dietary plants (Capsicum frutescens L. var. facilulatum, Brassica oleacera L. var. italica, Brassica oleacera L. var. butyris and Basilicum polystachyon (L.) Moench.) to improve the activity of commonly used antibiotics against MDR Gram-negative bacteria expressing active efflux pumps.
METHODS
The qualitative phytochemical screening of the plant extracts was performed using standard methods whilst the antibacterial activity was performed by broth micro-dilution method.
RESULTS
All the studied plant extracts revealed the presence of alkaloids, phenols, flavonoids, triterpenes and sterols. The minimal inhibitory concentrations (MIC) of the studied extracts ranged from 256-1024 μg/mL. Capsicum frutescens var. facilulatum extract displayed the largest spectrum of activity (73%) against the tested bacterial strains whilst the lower MIC value (256 μg/mL) was recorded with Basilicum polystachyon against E. aerogenes ATCC 13048 and P. stuartii ATCC 29916. In the presence of PAβN, the spectrum of activity of Brassica oleacera var. italica extract against bacteria strains increased (75%). The extracts from Brassica oleacera var. butyris, Brassica oleacera var. italica, Capsicum frutescens var. facilulatum and Basilicum polystachyon showed synergistic effects (FIC ≤ 0.5) against the studied bacteria, with an average of 75.3% of the tested antibiotics.
CONCLUSION
These results provide promising information for the potential use of the tested plants alone or in combination with some commonly used antibiotics in the fight against MDR Gram-negative bacteria.
背景
多药耐药（MDR）细菌的持续传播，部分原因是外排泵的大量存在，极大地降低了抗生素武器库的疗效，增加了治疗失败的频率。因此，寻找新的化合物来增强常用抗生素的疗效非常重要。本研究旨在评估四种喀麦隆食用植物（辣椒，芥菜，芥菜和罗勒）甲醇提取物的能力，以提高表达主动外排泵的 MDR 革兰氏阴性菌对抗生素的活性。
方法
采用标准方法对植物提取物进行定性植物化学筛选，采用肉汤微量稀释法进行抗菌活性测定。
结果
所有研究的植物提取物均显示存在生物碱，酚类，类黄酮，三萜类和甾体。研究提取物的最小抑菌浓度（MIC）范围为 256-1024μg/mL。辣椒 var. facilulatum 提取物对测试细菌菌株显示出最大的活性谱（73％），而罗勒提取物对大肠埃希菌 ATCC 13048 和肺炎克雷伯菌 ATCC 29916 的 MIC 值最低（256μg/mL）。在 PAβN 的存在下，芥菜 var. italica 提取物对细菌菌株的活性谱增加（75％）。芥菜 var. butyris，芥菜 var. italica，辣椒 var. facilulatum 和罗勒提取物对研究细菌表现出协同作用（FIC≤0.5），平均有 75.3％的测试抗生素。
结论
这些结果为单独使用或与一些常用抗生素联合使用测试植物对抗 MDR 革兰氏阴性菌提供了有希望的信息。
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