Zhou Xia, Zhou Fan, Jiang Hua, Hou Jian
Department of Hematology, Changzheng Hospital, the Second Military Medical University, Shanghai 200003, China.
Zhonghua Xue Ye Xue Za Zhi. 2007 May;28(5):318-22.
To investigate the repression of c-myc induced by Blimp-1 in 2-methoxyestradiol(2ME2)-mediated differentiation of multiple myeloma cells.
CZ-1 and LP-1 myeloma cells lines were exposed to 0.5 micromol/L of 2ME2 and 0.5 micromol/L of 2ME2 + antisense oligonucleotides (ASODN) for 72 h. The effects on transcription of c-myc mRNA were studied by RT-PCR. The c-myc protein was assayed with Western blot. The changes of the cell lines in morphology, expression of surface CD49e and quantity of immunoglobin light chain secretion in the supernatant were studied.
Incubation of the cells with 0.5 micromol/L of 2ME2 could up-regulated Blimp-1 expression and increase the expression of c-myc gene. In contrast, c-myc expression was decreased with Blimp-1 expression down-regulated by ASODN and the cell lines differentiation was arrested.
Blimp-1 could directly repress the expression of c-myc in 2ME2-mediated differentiation induction of multiple myeloma cells.
研究在2-甲氧基雌二醇（2ME2）介导的多发性骨髓瘤细胞分化过程中，Blimp-1对c-myc的抑制作用。
将CZ-1和LP-1骨髓瘤细胞系分别用0.5微摩尔/升的2ME2以及0.5微摩尔/升的2ME2 +反义寡核苷酸（ASODN）处理72小时。通过逆转录聚合酶链反应（RT-PCR）研究对c-myc mRNA转录的影响。用蛋白质免疫印迹法检测c-myc蛋白。研究细胞系在形态、表面CD49e表达以及上清液中免疫球蛋白轻链分泌量方面的变化。
用0.5微摩尔/升的2ME2处理细胞可上调Blimp-1表达并增加c-myc基因的表达。相反，当ASODN下调Blimp-1表达时，c-myc表达降低，细胞系分化停滞。
在2ME2介导的多发性骨髓瘤细胞分化诱导过程中，Blimp-1可直接抑制c-myc的表达。
