Liu Min, Wang Chuan-xin, Deng Xiao-mei, Wang Li-shui, Zhang Jian, Li Wei, Zheng Gui-xi, Wang Jin-feng
Department of Clinical Laboratory, Qilu Hospital, Shandong University, Jinan 250012, China.
Zhonghua Liu Xing Bing Xue Za Zhi. 2007 May;28(5):487-90.
To evaluate the diagnostic applicability of human papillomavirus (HPV) liquid chip assay which is based on Luminex XMAP System, and perform a HPV epidemiologic study with the liquid chip in women of Shandong province.
To detect HPV genotypes on a 96-well plate with the liquid chip which can simultaneously detect and identify 26 common HPV genotypes in a total of 2925 cervical scrapes obtained from gynecological outpatients as well as to analyze the relationship between HPV types and different cervical diseases by studying the distribution of HPV genotypes and pathologic diagnosis.
Among 639 cases who performed pathologic/cytological and histological diagnoses, 184 cases are in group of normal cytology, 266 cases in group of, 77 cases in group of cervical intra-epithelial neoplasia (CIN) I, 7 cases in group of CIN I - II, 46 cases in group of CIN I - II, 46 cases in group of CIN I - II and 13 cases in group of cervical cancer. The overall incidence of HPV in our samples is 36.0% (1054/2925) and 23 types of all 26 types on liquid chip are found. The most common genotypes found are HPV-16 (26.75%), HPV-52 (25.75%), HPV-58 (10.47%), HPV-18 (8.87%) and HPV-11 (6.94%). Among all the positive types, 87.32% are high-risk HPV and 13.68% are low-risk HPV genotypes. Both single and multiple types are easily identified, showing 66.22% ( n = 698) single type and 33.78% ( n = 356) multiple types. Of all the 1054 HPV-positive cases, 261 (24.8%) is occupied by women 21 to 25 years of age and progressively lower by older age groups, reaching 4.9% by women between 51 to 67 years old. The incidence of HPV in our samples is 23.37%, 33.08%, 54.54%, 57.14%, 82.61%, 91.30% and 100% for normal cytology, inflammation,CIN I ,CIN I - II, CIN II ,CIN III, and carcinomas specimens, respectively. Infections with more that one virus are common, accounted for 4.89%, 7.14%, 18.18%, 28.57%, 41.30%, 43.37% and 38.46% for normal cytology, inflammation, CIN I, CIN I - II, CIN II, CIN III, and carcinomas specimens, respectively. Based on the criteria of histology and pathology, the sensitivity, specificity, positive-predictive value and negative-predictive value of HPV liquid chip assay for detecting all cases of CIN II, III are 88.57%, 76.63%, 68.89% and 92.16% respectively. Conclusion The common types of HPV infection are 16, 52, 58, 18, 11, 6, 56 and 31. The HPV-positive rate increased along with the increase of grading on cervical lesions. There are more younger women among all the HPV-positive ones. Multiplex HPV genotyping by liquid chip appears to be highly suitable for diagnostic screening as well as the conduction of large-scale epidemiological studies.
评估基于Luminex XMAP系统的人乳头瘤病毒（HPV）液相芯片检测法的诊断适用性，并运用该液相芯片对山东省女性进行HPV流行病学研究。
采用可同时检测和鉴定26种常见HPV基因型的液相芯片，在96孔板上对2925例妇科门诊患者的宫颈刮片进行HPV基因分型检测，并通过研究HPV基因型分布与病理诊断结果，分析HPV类型与不同宫颈疾病之间的关系。
在639例进行了病理/细胞学及组织学诊断的病例中，正常细胞学组184例，炎症组266例，宫颈上皮内瘤变（CIN）I级组77例，CIN I-II级组7例，CIN II级组46例，CIN III级组46例，宫颈癌组13例。样本中HPV总体感染率为36.0%（1054/2925），液相芯片上26种HPV基因型中的23种被检测到。最常见的基因型为HPV-16（26.75%）、HPV-52（25.75%）、HPV-58（10.47%）、HPV-18（8.87%）和HPV-11（6.94%）。在所有阳性类型中，高危型HPV占87.32%，低危型HPV基因型占13.68%。单型和多型感染均易于识别，单型感染占66.22%（n=698），多型感染占33.78%（n=356）。在1054例HPV阳性病例中，21至25岁女性占261例（24.8%），且随年龄增长逐渐降低，51至67岁女性占4.9%。正常细胞学、炎症、CIN I、CIN I-II、CIN II、CIN III和癌组织样本中HPV感染率分别为23.37%、33.08%、54.54%、57.14%、82.61%、91.30%和100%。多种病毒感染较为常见，正常细胞学、炎症、CIN I、CIN I-II、CIN II、CIN III和癌组织样本中分别占4.89%、7.14%、18.18%、28.57%、41.30%、43.37%和38.46%。基于组织学和病理学标准，HPV液相芯片检测法检测所有CIN II、III级病例的灵敏度、特异度、阳性预测值和阴性预测值分别为88.57%、76.63%、68.89%和92.16%。结论HPV感染的常见类型为16、52、58、18、11、6、56和31。HPV阳性率随宫颈病变分级增加而升高。HPV阳性患者中年轻女性居多。液相芯片多重HPV基因分型非常适合诊断筛查及大规模流行病学研究。
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