基于Luminex XMAP的新型DNA液体芯片对人乳头瘤病毒基因分型的评估

[Evaluation of genotyping of human papilomavirus by a new DNA liquid chip based on Luminex XMAP].

作者信息

Deng Xiao-mei, Liu Min, Zheng Gui-xi, Wang Chuan-xin, Zhang Jian, Li Wei

机构信息

Department of clinical laboratory, Qilu hospital of Shandong University, Jinan 250012, China.

出版信息

Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2009 Dec;23(6):476-8.

PMID:20718362

Abstract

OBJECTIVE

To evaluate the diagnostic value of Luminex XMAP liquid chip for HPV detection.

METHODS

Detect HPV DNA with the liquid chip and HC II in 264 cases, including 231 of cervical cancer and 33 of cervical scrapes. The accordance of two methods were compared and DNA sequencing was performed in conflicting samples and single (infection samples.

RESULTS

There is an excellent agreement between the methods. The overall incidence of HPV was 82.95%, the most common genotypes were HPV 16, 52, 58, 18, 11, 31, 6, 39, 33, 56, 70. Among all the positive types, 117 were single type and 102 were multiple types, and 87.43% were high-risk HPV genotypes and 12.57% were low-risk genotypes. Based on the criteria of histology and pathology, the sensitivity, specificity, positive and negative-predictive value of HPV liquid chip and HC II for detecting all cases of CIN II, III and carcinomas were respectively 93.07%, 87.88%, 98.17%, 64.44% and 94.81%, 87.88%, 98.21%, 70.73%.

CONCLUSION

The common types of HPV infection are 16, 52, 58, 18, 11, 6, 56 and 31. Multiplex HPV genotyping by Luminex XMAP liquid chip appears to be highly suitable for diagnostic screening and large-scale epidemiological studies.

摘要

目的

评估Luminex XMAP液相芯片检测人乳头瘤病毒（HPV）的诊断价值。

方法

采用液相芯片和杂交捕获二代（HC II）技术检测264例样本中的HPV DNA，其中包括231例宫颈癌样本和33例宫颈刮片样本。比较两种方法的一致性，并对结果不一致的样本及单一感染样本进行DNA测序。

结果

两种方法一致性良好。HPV总感染率为82.95%，最常见的基因型为HPV 16、52、58、18、11、31、6、39、33、56、70。在所有阳性类型中，117例为单一型，102例为多重型，高危HPV基因型占87.43%，低危基因型占12.57%。以组织学和病理学标准为依据，HPV液相芯片和HC II检测所有CIN II、III级病变及癌病例的敏感性、特异性、阳性预测值和阴性预测值分别为93.07%、87.88%、98.17%、64.44%和94.81%、87.88%、98.21%、70.73%。