[柔红霉素和阿糖胞苷对NB4细胞系的作用]

[Effect of daunorubicin and cytarabine on cell line NB4].

作者信息

Luo Zhong-Guang, Zhong Lu, Chen Fang-Yuan, Wang Hai-Rong, Ouyang Ren-Rong

机构信息

Department of Hematology, Renji Hospital, Medical College of Shanghai Jiaotong University, Shanghai 200001, China.

出版信息

Zhonghua Xue Ye Xue Za Zhi. 2007 Apr;28(4):247-9.

PMID:17877202

Abstract

OBJECTIVE

To explore whether daunorubicin (DNR) combined with cytosine arabinoside (Ara-C) and DNR alone have similar effect on acute promyelocytic leukemia (APL) cell line NB4 and acute myeloblastic leukemia cell line HL-60 in vitro.

METHODS

Cell morphology, cells viability, and cell apoptosis (Annexin-V by flow cytometry assay) were analysed.

RESULTS

After incubation with DNR plus Ara-C for 24 hours,NB4 cell viability [(36.75 +/- 3.82)%] (n = 6) and cell apoptosis rate [(21.24 +/- 5.82)%] (n = 3) did not change significantly compared to that treated with DNR alone for 24 hours [(35.73 + 6.28 )%, (22.55 +/- 3.26)%, respectively] (P > 0.05). However, HL-60 cell viability [(67.17 +/- 2.07)%] and cell apoptosis rate [(48.05 +/- 0.92)%] changed significantly in DNR plus Ara-C group compared with DNR alone [(63.31 +/- 1.80)% ,(41.51 +/- 0.89)%, respectively] (P < 0.01 and < 0.05, respectively).

CONCLUSION

DNR plus Ara-C and DNR alone have similar effect on NB4 cells, but have different effect on HL-60 cells.

摘要

目的

探讨柔红霉素（DNR）联合阿糖胞苷（Ara-C）与单独使用DNR对急性早幼粒细胞白血病（APL）细胞系NB4和急性髓细胞白血病细胞系HL-60的体外作用是否相似。

方法

分析细胞形态、细胞活力及细胞凋亡情况（采用流式细胞术检测膜联蛋白-V）。

结果

与单独使用DNR处理24小时相比，DNR联合Ara-C处理24小时后，NB4细胞活力[（36.75±3.82）%]（n = 6）及细胞凋亡率[（21.24±5.82）%]（n = 3）无明显变化[分别为（35.73 + 6.28）%，（22.55±3.26）%]（P>0.05）。然而，与单独使用DNR相比，DNR联合Ara-C组HL-60细胞活力[（67.17±2.07）%]及细胞凋亡率[（48.05±0.92）%]有明显变化[分别为（63.31±1.80）%，（41.51±0.89）%]（P分别<0.01和<0.05）。