Bentzel David E, Lescano Andrés G, Lucas Carmen, Bacon David J
Laboratory Animal Program, Naval Medical Research Center Detachment, Lima, Peru.
J Am Assoc Lab Anim Sci. 2007 Sep;46(5):50-3.
Infections of Trypanoxyuris spp. pinworms in Aotus nancymae and other New World primates are typically subclinical, but infection during experimental use could confound interpretation of experimental data. Further, Trypanoxyuris species are highly infective, and rapid diagnosis is important to prevent an outbreak in the animal colony. This study sought to determine whether a fecal flotation technique was sensitive enough to replace the perianal tape test for diagnosis of Trypanoxyuris spp., thereby reducing stress to the animal and sample collection time. On days 0 and 3, we collected fecal samples from 45 animals confirmed to be infected with Trypanoxyuris spp. by perianal tape testing. Fecal samples were evaluated by both a commercial analysis system and by sucrose flotation with centrifugation. For both detection methods, no significant difference in sensitivity was detected between tests conducted on day 0 versus day 3. The sensitivity of repeated commercial tests was 80%, significantly higher than the 60% for sucrose flotation. The commercial test was significantly more sensitive than sucrose flotation, indicating that the commercial system was a better method for detecting Trypanoxyuris spp. However, sensitivity of only 80% confers a considerable risk of false negatives, thereby potentially delaying treatment and further contributing to environmental contamination. In our opinion, neither method of fecal analysis was a suitable replacement for the perianal tape test to diagnose Trypanoxyuris spp. in owl monkeys.
南希夜猴（Aotus nancymae）及其他新大陆灵长类动物感染曲尾线虫属（Trypanoxyuris spp.）蛲虫通常为亚临床感染，但在实验过程中感染可能会混淆实验数据的解读。此外，曲尾线虫属具有高度传染性，快速诊断对于预防动物群体中的疫情爆发很重要。本研究旨在确定粪便漂浮技术是否足够灵敏，能够替代肛周胶带试验来诊断曲尾线虫属，从而减轻动物的应激并缩短样本采集时间。在第0天和第3天，我们从45只经肛周胶带试验确诊感染曲尾线虫属的动物身上采集粪便样本。粪便样本通过商业分析系统和蔗糖漂浮离心法进行评估。对于这两种检测方法，在第0天和第3天进行的测试之间未检测到敏感性的显著差异。重复进行的商业测试的敏感性为80%，显著高于蔗糖漂浮法的60%。商业测试比蔗糖漂浮法明显更灵敏，表明商业系统是检测曲尾线虫属的更好方法。然而，仅80%的敏感性存在相当大的假阴性风险，从而可能延迟治疗并进一步导致环境污染。我们认为，在夜猴中诊断曲尾线虫属时，这两种粪便分析方法都不适宜替代肛周胶带试验。
