Quemelo Paulo Roberto Veiga, Sbragia Lourenço, Peres Luiz Cesar
Department of Pathology, Ribeirão Preto School of Medicine, University of São Paulo, Ribeirão Preto, SP, Brazil.
Int J Exp Pathol. 2007 Oct;88(5):337-42. doi: 10.1111/j.1365-2613.2007.00536.x.
The presence of heterotopic brain tissue in the lung is a rare abnormality. The cases reported thus far are usually associated with neural tube defects (NTD). As there are no reports of experimental models of NTD that present this abnormality, the objective of the present study was to develop a surgical method of brain tissue heterotopia in the lung. We used 24 pregnant Swiss mice divided into two groups of 12 animals each, denoted 17GD and 18GD according to the gestational day (GD) when caesarean section was performed to collect the fetuses. Surgery was performed on the 15th GD, one fetus was removed by hysterectomy and its brain tissue was cut into small fragments and implanted in the lung of its litter mates. Thirty-four live fetuses were obtained from the 17GD group. Of these, eight (23.5%) were used as control (C), eight (23.5%) were sham operated (S) and 18 (52.9%) were used for pulmonary brain tissue implantation (PBI). Thirty live fetuses were obtained from the females of the 18GD group. Of these, eight (26.6%) were C, eight (26.6%) S and 14 (46.6%) were used for PBI. Histological examination of the fetal trunks showed implantation of GFAP-positive brain tissue in 85% of the fetuses of the 17GD group and in 100% of those of the 18GD group, with no significant difference between groups for any of the parameters analysed. The experimental model proved to be efficient and of relatively simple execution, showing complete integration of the brain tissue with pulmonary and pleural tissue and thus representing a model that will permit the study of different aspects of cell implantation and interaction.
肺内异位脑组织是一种罕见的异常情况。迄今为止报道的病例通常与神经管缺陷（NTD）有关。由于尚无出现这种异常情况的NTD实验模型的报道，本研究的目的是开发一种在肺内形成脑组织异位的手术方法。我们使用了24只怀孕的瑞士小鼠，分为两组，每组12只动物，根据剖腹产收集胎儿时的妊娠天数（GD）分别标记为17GD和18GD。手术在妊娠第15天进行，通过子宫切除术取出一只胎儿，将其脑组织切成小碎片并植入其同窝仔鼠的肺中。从17GD组获得了34只活胎儿。其中，8只（23.5%）用作对照（C），8只（23.5%）进行假手术（S），18只（52.9%）用于肺脑组织植入（PBI）。从18GD组的母鼠中获得了30只活胎儿。其中，8只（26.6%）为C，8只（26.6%）为S，14只（46.6%）用于PBI。对胎儿躯干的组织学检查显示，17GD组85%的胎儿和18GD组100%的胎儿中有GFAP阳性脑组织植入，在所分析的任何参数上两组之间均无显著差异。该实验模型被证明是有效的且执行相对简单，显示出脑组织与肺和胸膜组织完全整合，因此代表了一种可用于研究细胞植入和相互作用不同方面的模型。
