Diez-Pardo J A, Mariño J M, Baoquan Q, Delgado-Baeza E, Fernáneez A, Morales M C, Tovar J A
Department of Paediatric Surgery, Hospital La Paz, Madrid, Spain.
Eur J Pediatr Surg. 1995 Aug;5(4):198-202. doi: 10.1055/s-2008-1066204.
We report on our experience in the experimental induction of Neural Tube Defects (NTD) in the foetal rat by maternal administration of retinoic acid. The teratogen diluted in olive oil was administered in a single intragastric dose (125 mg/kg body weight) to pregnant rats (n = 31) on the 10th day of gestation. Pure olive oil was given to control rats (n = 9). The foetuses were recovered by caesarian section on the 20th day and prepared for morphological investigation. We have studied 201 experimental and 82 control animals. There were NTD in 36.3% of experimental foetuses and none in the control ones. Sacral dysraphism was the most frequent defect but we also observed Arnold Chiari malformations and crowding of the bony limits by an enlarged neural axis. Other associated malformations found were: craneofacial (78.1%), caudal (80%), anorectal (31.4%), and limb defects (89.5%). This simple and inexpensive model may allow us to gain a better knowledge of the biology in the foetus with NTD.
我们报告了通过给孕鼠母体施用视黄酸在胎鼠中实验诱导神经管缺陷（NTD）的经验。将稀释于橄榄油中的致畸剂以单次胃内剂量（125毫克/千克体重）给予妊娠第10天的孕鼠（n = 31）。给对照鼠（n = 9）给予纯橄榄油。在第20天通过剖腹产取出胎儿并准备进行形态学研究。我们研究了201只实验动物和82只对照动物。36.3%的实验胎儿出现神经管缺陷，而对照胎儿未出现。骶部脊柱裂是最常见的缺陷，但我们也观察到阿诺德·基亚里畸形以及神经轴增粗导致的骨界拥挤。发现的其他相关畸形有：颅面畸形（78.1%）、尾部畸形（80%）、肛门直肠畸形（31.4%）和肢体缺陷（89.5%）。这个简单且成本低廉的模型可能使我们更好地了解患有神经管缺陷的胎儿的生物学特性。
