Foulger D, Errington J
Sir William Dunn School of Pathology, University of Oxford, UK.
Mol Microbiol. 1991 Jun;5(6):1363-73. doi: 10.1111/j.1365-2958.1991.tb00783.x.
The spoVJ gene of Bacillus subtilis encodes a 36 kDa protein and is expressed only in the mother cell. spoVJ has an interesting pattern of regulation during sporulation because it is expressed from sequentially activated promoters. These promoters, designated P1 and P2, are under the control of different sigma factors, sigma E and sigma K, which become active at separate times during sporulation. Removal of promoter P1, leaving promoter P2 active, resulted in about a 30-minute delay in the formation of heat-resistant spores and demonstrated that the expression of spoVJ from both promoters is essential for normal sporulation. A comparison is made between the sequences of the spoVJ promoters and the promoters of other genes dependent upon sigma E and sigma K.
枯草芽孢杆菌的spoVJ基因编码一种36 kDa的蛋白质，且仅在母细胞中表达。spoVJ在芽孢形成过程中具有有趣的调控模式，因为它是从顺序激活的启动子表达的。这些启动子，命名为P1和P2，受不同的σ因子σE和σK的控制，它们在芽孢形成过程中的不同时间变得活跃。去除启动子P1，使启动子P2活跃，导致耐热孢子形成延迟约30分钟，并表明来自两个启动子的spoVJ表达对于正常芽孢形成至关重要。对spoVJ启动子的序列与其他依赖于σE和σK的基因的启动子序列进行了比较。
