Zahn L, Freund R, Noack R, Erhardt V, Augustin W
Central Institute of Nutrition in Potsdam-Rehbrücke, Federal Republic of Germany.
Nahrung. 1991;35(6):591-602. doi: 10.1002/food.19910350606.
Body composition data estimated for normal young, normal adult, and adult obese Wistar rats by a tritiated water (THO) dilution procedure (b. wt. range 119-237 g, n = 31; 305-597 g, n = 43; 274-381 g, n = 6) were compared with the results of whole body analysis. As for the predicted total body lipid (TBL), the content was calculated from the virtual THO-dilution space estimated by a simple micro-method and a regression equation between TBL [%] and DM [%] (dry matter) content (Part 1. Relations between directly measured major body components. Nahrung 35 (1991) 581. Due to different degrees of overestimations in the animal groups examined, the THO-space can be corrected via subtraction of 3.8%, 2.2% and 1.1% in the sequence young, obese, and adult groups. The derived TBL [%] data show a non-significant deviation of less than 1 g/100 g b. wt. in comparison to the three group means after direct lipid determinations. In the best case, i. e. after the additional elimination of extremely overestimated values for total body water (outliers) the combined tritium-derived lipid values (y) are correlated with the lipid data obtained analytically (x), both as proportion of the body weight, by the regression equation y = 0.848 + 0.963 x, r = 0.985, Sy.x = 1.77, n = 58. For a group of young rats (n = 14) after a pre-experimental supplementation with a sucrose solution, TBL values, not significantly different from analytically determined ones, could be calculated without any corrections.
采用氚水（THO）稀释法对正常幼年、正常成年和成年肥胖Wistar大鼠（体重范围119 - 237 g，n = 31；305 - 597 g，n = 43；274 - 381 g，n = 6）的身体成分数据进行了估算，并与全身分析结果进行了比较。至于预测的全身脂质（TBL），其含量是根据一种简单的微量方法估算的虚拟THO稀释空间以及TBL [%]与DM [%]（干物质）含量之间的回归方程计算得出的（第1部分。直接测量的主要身体成分之间的关系。《营养》35 (1991) 581）。由于在所研究的动物组中存在不同程度的高估，THO空间可通过在幼年、肥胖和成年组中依次减去3.8%、2.2%和1.1%来进行校正。与直接脂质测定后的三组平均值相比，得出的TBL [%]数据显示出小于1 g/100 g体重的非显著偏差。在最佳情况下，即额外剔除全身水分的极度高估值（异常值）后，氚衍生的脂质组合值（y）与通过分析获得的脂质数据（x）相关，两者均以体重比例表示，回归方程为y = 0.848 + 0.963x，r = 0.985，Sy.x = 1.77，n = 58。对于一组在实验前补充蔗糖溶液的幼年大鼠（n = 14），无需任何校正即可计算出与分析测定值无显著差异的TBL值。
