Rondello Bonatti Taís, Bueno Franco Regina Maura, Cantusio Neto Romeu
Parasitology Department, Biology Institute, State University of Campinas, SP Brazil PO Box 6109, CEP:13083-970.
J Water Health. 2007 Dec;5(4):609-14. doi: 10.2166/wh.2007.006.
Giardia spp. and Cryptosporidium spp. are recognized worldwide as highly infectious protozoan parasites that can cause severe gastrointestinal disease in humans and animals. The detection of these pathogens in activated sludge samples becomes interesting since there is an increasing trend for the use of sewage sludge (biosolids) in agriculture. A total of 22 samples were collected and evaluated by means of Centrifugal - Concentration, followed or not followed by a purification process (ether clarification and sucrose flotation). Student t tests for comparison of the two procedures indicated a higher recovery rate of Giardia cysts with Centrifugal - Concentration; with regard to Cryptosporidium oocysts, no significant differences were found between the two methods, as only two samples were positive. The Centrifugal - Concentration procedure was shown to be the simplest and cheapest to perform, as emphasized by the efficiency recovery results.
贾第虫属和隐孢子虫属被公认为是极具传染性的原生动物寄生虫，可在人和动物中引起严重的胃肠道疾病。鉴于农业中使用污水污泥（生物固体）的趋势日益增加，在活性污泥样本中检测这些病原体变得很有意义。总共收集了22个样本，并通过离心浓缩法进行评估，之后可选择或不进行纯化过程（乙醚澄清和蔗糖浮选）。用于比较这两种方法的学生t检验表明，离心浓缩法对贾第虫包囊的回收率更高；对于隐孢子虫卵囊，由于只有两个样本呈阳性，两种方法之间未发现显著差异。如效率回收结果所强调的，离心浓缩法被证明是最简单且最便宜的操作方法。
