Fan JinHui, Li WenQing, Dong XiuChun, Guo Wei, Shu HuaiRui
College of Forestry, Shandong Agricultural University, Taian 271018, China.
Sci China C Life Sci. 2007 Oct;50(5):676-89. doi: 10.1007/s11427-007-0083-4.
MADS-box genes are involved in floral organ development. Here we report that an AGL6(Agamous-like 6)-like MADS-box gene, HoAGL6, was isolated from Hyacinthus orientalis L. Expression pattern analysis demonstrated that HoAGL6 transcript was detected in inflorescence buds, tepals, carpels and ovules, but not in stamina, leaves or scales. Transgenic Arabidopsis plants ectopically expressing HoAGL6 exhibited novel phenotypes of significantly reduced plant size, extremely early flowering, and losing inflorescence indeterminacy. In addition, wide homeotic conversion of sepals, petals, and leaves into carpel-like or ovary structures, and disappearance or number reduction of stamens in 35S::HoAGL6 Arabidopsis plants were also observed. RT-PCR analysis indicated that the expressions of flowering time gene SOC1 and flower meristem identity gene LFY were significantly up-regulated in 35S::HoAGL6 transgenic Arabidopsis plants, and the expression levels of floral organ identity genes AG and SEP1 in leaves were also elevated. These results indicated that HoAGL6 was involved in the regulation of flower transition and flower organ formation.
MADS盒基因参与花器官的发育。在此我们报道，从风信子中分离出一个类似AGL6（类AGAMOUS 6）的MADS盒基因HoAGL6。表达模式分析表明，在花序芽、花被片、心皮和胚珠中检测到HoAGL6转录本，但在雄蕊、叶片或鳞片中未检测到。异位表达HoAGL6的转基因拟南芥植株表现出植株大小显著减小、极早开花以及花序失去无限生长特性等新表型。此外，在35S::HoAGL6拟南芥植株中还观察到萼片、花瓣和叶片广泛地同源异型转化为心皮状或子房结构，以及雄蕊消失或数量减少。RT-PCR分析表明，在35S::HoAGL6转基因拟南芥植株中，开花时间基因SOC1和花分生组织特征基因LFY的表达显著上调，叶片中花器官特征基因AG和SEP1的表达水平也升高。这些结果表明，HoAGL6参与了花转变和花器官形成的调控。
