Wen Wen, Wan Junfen, Cao Xuejun, Xia Jinan
State Key Laboratory of Bioreactor Engineering, Department of Biochemical Engineering, East China University of Science & Technology, 130 Meilong Rd., Shanghai, China 200237.
Biotechnol Prog. 2007 Sep-Oct;23(5):1124-9. doi: 10.1021/bp070114f. Epub 2007 Sep 19.
A novel light-sensitive and cation-exchange copolymer (PNBCC) has been synthesized by random copolymerization of chlorophyllin sodium copper salt, crylic acid, n-butyl acrylate, and N-isopropylacrylamide. The PNBCC copolymer showed reversible dissolution and could be precipitated by 488 nm laser irradiation with the least light density of 1.70 x 10(5) W/m(2). By optimizing the ratio of monomers, pH, and ion concentration, over 95% copolymer was recovered by laser irradiation. The copolymer was used to purify lysozyme as light-sensitive cation exchanger, and its adsorption matched a Langmuir adsorption isotherm with maximum adsorption capacity of 98,900 U/g and dissociated constant of 852 U/mL. By applying the copolymer to the separation of lysozyme from egg white, the specific activity of lysozyme was improved from 399 to 6346 U/mg and the recovery of lysozyme achieved 81.3%.
通过叶绿素铜钠盐、丙烯酸、丙烯酸丁酯和N-异丙基丙烯酰胺的无规共聚合成了一种新型的光敏阳离子交换共聚物（PNBCC）。PNBCC共聚物表现出可逆溶解，并且可以在最低光密度为1.70×10(5) W/m(2)的488 nm激光照射下沉淀。通过优化单体比例、pH值和离子浓度，超过95%的共聚物可通过激光照射回收。该共聚物用作光敏阳离子交换剂来纯化溶菌酶，其吸附符合朗缪尔吸附等温线，最大吸附容量为98,900 U/g，解离常数为852 U/mL。通过将该共聚物应用于从蛋清中分离溶菌酶，溶菌酶的比活性从399提高到6346 U/mg，溶菌酶的回收率达到81.3%。
