Kampmann Eric, Mey Jörg
Institut für Biologie II, RWTH-Aachen, Kopernikusstrasse 16, D-52074 Aachen, Germany.
Neurosci Lett. 2007 Oct 9;426(1):18-22. doi: 10.1016/j.neulet.2007.07.039. Epub 2007 Aug 6.
The transcriptional activator retinoic acid (RA) is a regulator of neural development and regeneration. Synergistic effects with brain-derived neurotrophic factor suggested that RA influences neurotrophin signaling. To test this hypothesis RA was administered systemically to E17 chick embryos, and retinas were prepared 12h and 24h later to measure mRNA or protein expression. While there was no significant influence on activation of Akt, CREB and STAT-3, RA-treatment caused elevated levels of Erk-phosphorylation, a kinase involved in Trk signaling. A small but significant increase in the expression of TrkB mRNA and protein was observed but no significant change in TrkA, TrkC and p75 expression.
转录激活因子视黄酸（RA）是神经发育和再生的调节因子。与脑源性神经营养因子的协同作用表明RA影响神经营养因子信号传导。为了验证这一假设，将RA全身给予E17期鸡胚，并在12小时和24小时后制备视网膜以测量mRNA或蛋白质表达。虽然RA处理对Akt、CREB和STAT-3的激活没有显著影响，但导致参与Trk信号传导的激酶Erk磷酸化水平升高。观察到TrkB mRNA和蛋白质表达有小幅但显著的增加，但TrkA、TrkC和p75表达没有显著变化。
