DNA与Cu(II)-柚皮苷复合物相互作用的电化学和光谱表征
Electrochemical and spectroscopic characterization of the interaction between DNA and Cu(II)-naringin complex.
作者信息
Mello Lucilene D, Pereira Regina M S, Sawaya Alexandra C H F, Eberlin Marcos N, Kubota Lauro T
机构信息
Instituto de Química, UNICAMP, P.O. Box 6154, 13083-970 Campinas, SP, Brazil.
出版信息
J Pharm Biomed Anal. 2007 Dec 21;45(5):706-13. doi: 10.1016/j.jpba.2007.08.007. Epub 2007 Aug 14.
In this work the interaction of DNA and the flavonoid-transition metal complex (Cu(II)-naringin complex) is characterized. The interaction was evaluated by using electrochemical ssDNA- and dsDNA-based biosensors and the results were supported by UV, CD and (1)H NMR data. In the electrochemical method, changes in the oxidation peak of the guanine and adenine bases obtained by square wave voltammetry (SWV) showed evidence of the interaction. The variations of the spectroscopic characteristics of DNA and Cu(II)-naringin complex in aqueous medium demonstrated that the predominant interaction mode may be by intercalation. Cu(II)-naringin complex interacts to dsDNA probably via N(7) of guanine site.
在本研究中，对DNA与类黄酮 - 过渡金属配合物（Cu(II)-柚皮苷配合物）之间的相互作用进行了表征。通过基于单链DNA和双链DNA的电化学生物传感器评估了这种相互作用，紫外光谱、圆二色光谱和（1）H核磁共振数据也证实了该结果。在电化学方法中，通过方波伏安法（SWV）获得的鸟嘌呤和腺嘌呤碱基氧化峰的变化证明了这种相互作用的存在。DNA和Cu(II)-柚皮苷配合物在水性介质中的光谱特征变化表明，主要的相互作用模式可能是嵌入作用。Cu(II)-柚皮苷配合物可能通过鸟嘌呤位点的N(7)与双链DNA相互作用。
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