KCNQ1/KCNE1钾通道和P2Y4受体在大鼠螺旋韧带边缘细胞顶端膜从出生时起就共同表达。
KCNQ1/KCNE1 K+ channel and P2Y4 receptor are co-expressed from the time of birth in the apical membrane of rat strial marginal cells.
作者信息
Hur Dong Gu, Lee Jun Ho, Oh Seung-Ha, Kim Young Ho, Lee Jin Hee, Shin Dong Hoon, Chang Sun O, Kim Chong-Sun
机构信息
Department of Otorhinolaryngology-Head and Neck Surgery, Gyeongsang National University Hospital, Jinju, Korea.
出版信息
Acta Otolaryngol Suppl. 2007 Oct(558):30-5. doi: 10.1080/03655230701624830.
CONCLUSION
KCNQ1/KCNE1 K(+) channels and P2Y(4) receptors are expressed in the apical membrane of rat strial marginal cells from postnatal day 1 (P1) and maintained throughout development.
OBJECTIVES
The purpose of the present study was to investigate the developmental expression of KCNQ1/KCNE1 K(+) channel and of P2Y(4), which is an important metabotropic regulator of KCNQ1/KCNE1 K(+) channel in strial marginal cells.
MATERIALS AND METHODS
Sprague-Dawley rats at different stages of development (P1, P3, P5, P7, P14, and P21) were studied. The spiral ligament with the stria vascularis was detached from the cartilaginous or bony cochlea and prepared for a voltage-sensitive vibrating probe and immunohistochemistry.
RESULTS
Chromanol 293B, a blocker of KCNQ1/KCNE1 K(+) channel, inhibited short-circuit currents (I ( sc )) from P1 to P21. Similarly, I ( sc ) were found to be decreased by uridine 5'-triphosphate at all ages. The antagonist profiles indicated that the apical P2Y receptor is P2Y(4) subtype. KCNQ1, KCNE1, and P2Y(4) were immunolocalized in the apical region of stria vascularis at P1.
结论
KCNQ1/KCNE1钾通道和P2Y(4)受体在出生后第1天（P1）大鼠螺旋缘细胞的顶端膜中表达，并在整个发育过程中持续存在。
目的
本研究旨在探讨KCNQ1/KCNE1钾通道以及作为螺旋缘细胞中KCNQ1/KCNE1钾通道重要代谢型调节剂的P2Y(4)的发育表达情况。
材料与方法
研究了不同发育阶段（P1、P3、P5、P7、P14和P21）的Sprague-Dawley大鼠。将带有血管纹的螺旋韧带从软骨性或骨性耳蜗上分离下来，制备用于电压敏感振动探针和免疫组织化学检测的样本。
结果
KCNQ1/KCNE1钾通道阻滞剂Chromanol 293B在P从1到P21期间抑制短路电流（I(sc)）。同样，在所有年龄段，尿苷5'-三磷酸均使I(sc)降低。拮抗剂分析表明顶端P2Y受体为P2Y(4)亚型。在P1时，KCNQ1、KCNE1和P2Y(4)免疫定位在血管纹的顶端区域。
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