子宫珠蛋白在变应性鼻炎小鼠模型中的表达
Expression of uteroglobin in a murine model of allergic rhinitis.
作者信息
Won Tae-Bin, Quan Song-Hua, Rhee Chae-Seo, Min Yang-Gi, Lee Chul Hee
机构信息
Department of Otorhinolaryngology-Head and Neck Surgery, Hallym University College of Medicine, Seoul, Korea.
出版信息
Acta Otolaryngol Suppl. 2007 Oct(558):83-9. doi: 10.1080/03655230701624921.
CONCLUSION
We observed for the first time the expression of Uteroglobin (UGB) in the nasal mucosa of mice. The results of our study suggest that UGB may play an important role in the regulation of inflammation in allergic rhinitis (AR) as well as in the lower airway allergic inflammations.
OBJECTIVES
Uteroglobin is a protein secreted by epithelial lining of organs communicating with the external environment. Reports of its immunomodulatory effects in allergic disease have been made, but the true physiological role still remains to be elucidated. In this study we tried to observe the expression of UGB in the nasal mucosa of mice and determine its role in AR.
MATERIALS AND METHODS
Thirty BALB-c mice at 3 weeks of age (10 mice/group) were sensitized systemically by intraperitoneal ovalbumin injection and locally by ovalbumin inhalation. Control group were sensitized with PBS. Treatment group had intraperitoneal dexamethasone injection 1 hour before the initial sensitization while control and AR group were injected with PBS. Symptom scores, eosinophil counts, immunohistochemical staining as well as UGB mRNA expression in the nasal mucosa and lung tissue were analyzed.
RESULTS
The symptom scores and eosinophil counts between control and treatment group was significantly different from the AR group (P<0.01). On immunohistochemical staining, UGB was localized in the epithelium and submucosal gland of the nasal mucosa as well as in the epithelium of respiratory bronchioles. UGB mRNA expression of the nasal mucosa and lung tissue was decreased in the AR group compared to the control group (P=0.022). In the treatment group UGB expression was increased compared to the AR group (P=0.016). The results of IHC and mRNA expression in the lung tissue correlated with the results in the nasal mucosa.
结论
我们首次观察到子宫珠蛋白（UGB）在小鼠鼻黏膜中的表达。我们的研究结果表明，UGB可能在变应性鼻炎（AR）以及下呼吸道变应性炎症的炎症调节中发挥重要作用。
目的
子宫珠蛋白是由与外界环境相通的器官上皮衬里分泌的一种蛋白质。已有关于其在变应性疾病中免疫调节作用的报道，但其真正的生理作用仍有待阐明。在本研究中，我们试图观察UGB在小鼠鼻黏膜中的表达，并确定其在AR中的作用。
材料与方法
30只3周龄的BALB-c小鼠（每组10只）通过腹腔注射卵清蛋白进行全身致敏，并通过吸入卵清蛋白进行局部致敏。对照组用磷酸盐缓冲液（PBS）致敏。治疗组在初次致敏前1小时腹腔注射地塞米松，而对照组和AR组注射PBS。分析症状评分、嗜酸性粒细胞计数、免疫组织化学染色以及鼻黏膜和肺组织中UGB mRNA的表达。
结果
对照组和治疗组的症状评分和嗜酸性粒细胞计数与AR组有显著差异（P<0.01）。免疫组织化学染色显示，UGB定位于鼻黏膜的上皮和黏膜下腺以及呼吸细支气管的上皮。与对照组相比，AR组鼻黏膜和肺组织中UGB mRNA的表达降低（P=0.022）。与AR组相比，治疗组UGB的表达增加（P=0.016）。肺组织中免疫组织化学和mRNA表达的结果与鼻黏膜中的结果相关。
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